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자료유형
학술저널
저자정보
저널정보
환경독성보건학회 Environmental Analysis Health and Toxicology 한국환경독성학회지 제17권 제3호
발행연도
2002.9
수록면
239 - 243 (5page)

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Changes of plasma vitellogenin (VTG) and glutamate pyruvate transaminase (GPT) were examined for determining whether hepatocyte was damaged during the process of VTG induction in the juvenile rockfish, Sebastes schlegeli exposed to exogenous estrogen (estradiol-17β, E₂). Rockfishes were intraperitoneally injected with E₂, (5 mg/kg B.W.) in 70% ethanol and plasma sampling were extracted at 0, I, 3, 6, 9, 12, 15 days after E₂ administraton. VTG and GPT were then analyzed by SDS-PAGE and Reitman-Frankel method, respectively. VTG band was detected at a molecular weight position of 175 kDa on Day 3 after E₂, administration. This band became more distinct at 6 days, but its was gradually thinned with time course, and not detected at 15 days. GPT was suddenly increased at I days after E₂ administration and highest GPT was detected at 3 days. However, GPT was gradually decreased with time-course as the change of VTG. These results suggest that the process of VTG induction by exogenous E₂ damage to hepatocyte, and plasma GPT was temporarily increased in the juvenile rockfish.

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