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초록· 키워드
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The optimized RAPD-PCR conditions, that can be utilized as a basic information for analysis of the genetic characteristics were developed for genetic analysis of four mulberry varieties, named Milsung, Chungil, Suil, and Hansung using a primer, OPY15 (5'-AGTCGCCCTT-3') from Operon company. We tested several different factors for best PCR condition including concentrations of DNA, primer, MgCl₂, annealing temperature, number of PCR cycle, and presence/absence of pre-heating time at the begining of PCR reaction in the 25 μl volume. The best RAPD profiles were obtained using 50 ng of DNA, 1 μM of primer, 1.5 mM of MgCl₂, 45℃ of annealing temperature and an absence of pre-heating time. An establishment of the stable and reproducible RAPD-PCR conditions are expected to be useful for the subsequent RAPD-related investigation, such as genetic characterization of the mulberry varieties, re-establishment of phylogenetic relationships and development of new varieties.
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감사의 글
참 고 문 헌
참고문헌 (15)
참고문헌 신청
[학술지(정기간행물)] - / 1997 / Analysis of genetic Relationships among Glycine species using RAPDs 29 (3) : 308 ~ 317
[학술지(정기간행물)] - / 1995 / Reproducible amplification of RAPD markers from Vertebrate DNA 18 (5) : 856 ~ 860
[학술지(정기간행물)] - / 1995 / Protein and Lipid contents of Kangwon local soybean and their RAPDs 27 (3) : 215 ~ 220
[학술지(정기간행물)] - / 1994 / Optimization of PCR conditions for RAPD analysis in Radish Kor : 116 ~ 117
[학술지(정기간행물)] - / 1993 / Identification of apple cultivars using RAPD markers : 901 ~ 904
[학술지(정기간행물)] - / 1995 / Reproducible amplification of RAPD markers from Vertebrate DNA 18 (5) : 856 ~ 860
[학술지(정기간행물)] - / 1995 / Protein and Lipid contents of Kangwon local soybean and their RAPDs 27 (3) : 215 ~ 220
[학술지(정기간행물)] - / 1994 / Optimization of PCR conditions for RAPD analysis in Radish Kor : 116 ~ 117
[학술지(정기간행물)] - / 1993 / Identification of apple cultivars using RAPD markers : 901 ~ 904
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Genetic Variation of Korean Formica Ants using RAPD-PCR Analysis
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Mycoflora of Chicken-Viscera with Aid of RAPD Technique as a Tool for Confirmation
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단감나무로부터 분리한 탄저병 병원균 Colletotrichum spp.의 RAPD와 PCR-RFLP를 이용한 유연관계 분석
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Phylogenetic Relationships in the Genus Phintella (Araneae, Salticidae) Based on RAPD Analysis
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Differentiation of Fusarium oxysporum f. sp. fragariae Isolates by Random Amplified Polymorphic DNA (RAPD) Analysis
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UCI(KEPA) : I410-ECN-0101-2009-470-014221300