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The efficient fermentative production of L-threonine fermentation was achieved by using Escherichia coli MT201, transformed a plasmid carrying pyruvate carboxylase gene. It is an attempt to supply oxaloacetate to the L-threonine biosynthetic pathway. In order to improve the L-threonine productivity of E. coli MT201, a plasmid pPYC which is an expression vector of the pyruvate carboxylase gene of Corynebacterium glutamicum, was introduced. When E. coli MT/pPYC was incubated with medium containing only glucose as a carbon source, both the cell growth and L-threonine production were reduced, compared to the results from fermentation of E. coli MT201. In order to circumvent this effect, we attempted the addition of a mixed carbon source, composed of glucose and sodium citrate at a ratio of 1.5:3.5. It was shown that L-threonine production and cell growth (OD660) with E. coli MT/pPYC reached up to 75.7 g/l and 48, respectively, at incubation for 75 hr under fed-batch fermentation conditions. It is assumed that overproduction of L-threonine by anaplerotic pathway leads unbalance of TCA cycle and sodium citrate might play a role to recover normal TCA cycle.
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Abstract
재료 및 방법
결과 및 고찰
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감사의 글
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참고문헌 (13)
참고문헌 신청
[학술지(정기간행물)] - / 2000 / Enrichment of threonine content in Saccharomyces cerevisiae by pathway engineering 26 : 763 ~ 770
[학술지(정기간행물)] - / 1972 / Production of L- threonine by analog-resistant mutants : 1611 ~ 1621
[학술지(정기간행물)] - / 1998 / Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene 50 : 346 ~ 352
[학술지(정기간행물)] - / 1991 / Production of L-threonine by auxotrophs and analogue resistant mutants of Escherichia coli : 583 ~ 587
[학술지(정기간행물)] - / 1992 / Construction of L-threonine overproducing Escherichia coli by cloning of the threonine operon : 243 ~ 247
[학술지(정기간행물)] - / 1972 / Production of L- threonine by analog-resistant mutants : 1611 ~ 1621
[학술지(정기간행물)] - / 1998 / Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene 50 : 346 ~ 352
[학술지(정기간행물)] - / 1991 / Production of L-threonine by auxotrophs and analogue resistant mutants of Escherichia coli : 583 ~ 587
[학술지(정기간행물)] - / 1992 / Construction of L-threonine overproducing Escherichia coli by cloning of the threonine operon : 243 ~ 247
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UCI(KEPA) : I410-ECN-0101-2009-470-014448898