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자료유형
학술대회자료
저자정보
저널정보
한국가금학회 한국가금학회 정기총회 및 학술발표회 한국가금학회 제17차 정기총회 및 학술발표회 PROCEEDINGS
발행연도
2000.11
수록면
81 - 84 (4page)

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Effects of different photoperiod regimens on the cellular and humoral immunity in broiler chickens were studied (Exp 1). Total one hundred ninety two one-day-old commercial broiler chicks(Cobb×Cobb) were raised between constant lighting (CL) and intermittent lighting(1h light: 3h darkness(IL; lL: 3D)). Body weight, feed intake and feed conversion were measured for seven week. Peripheral blood and splenic lymphocyte activities were tested at 3 and 5 wk of age by performing a mitogen cellproliferation assay with a polyclonal T-cell mitogen, concanavalin A (Con A), and B-cell mitogen, lipopolysaccharide (LPS). To investigate the effect of photoperiod on the humoral immunity, chicks were immunized with sheep red blood cell (SRBC) and inactivated Newcastle disease virus (NDV) vaccine. Total immunoglobulin G (IgG) concentration was also determined. Diurnal change of melatonin was tested in sera. In experiment 2, 0.1㎖ melatonin were subcutaneously injected from three to five weeks old if immunomodulation effect of lighting regimen was due to the melatonin or not. Injections of melatonin were made at 0700h and the dosage was 10ng (M2), 100ng(M3), l㎍(M4) per bird daily, respectively. Control were quivalent injections of vehic1e(M1). Lymphocyte activities were tested and humoral immunities were examined at 5 weeks of age. Blood melatonin concentration was determined at Oh, 1h, 2h, and 3h posterior to injection at five weeks old. It was higher in CL chicks than IL chickens during the subsequent period of 3 to 5 wk of age. However, weight gain of chicks raised IL were significantly higher at 6 wk of age than CL(P<0.05). Antibody response to NDV was not affected by both photoperiod regimens and melatonin injection, whereas anti-SRBC titer and IgG concentration were enhanced. Lymphocyte activity of chickens raised under IL was sighificantly higher than those of chickens raised under CL. Melatonin injection also increased lymphocyte activity. When peripheral blood lymphocytes were used, proliferation response to LPS and Con A were significantly increased in M2 and M3, respectively. The results of this experiments suggest that IL improved host immune response and melatonin have immunomodulatory roles.

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UCI(KEPA) : I410-ECN-0101-2009-527-015208905