지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1997.11
- 수록면
- 381 - 386 (6page)
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초록· 키워드
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A series of experiments were conducted to compare the effects of various diluents in cold storage on the marbled sole, Limanda yokohamae sperm.
Various diluents of glucose, L. yokohamae serum, marine fish Ringer's solution, sodium citrate and Ca²+ free artificial seawater (ASW) (tris-HCl, pH 7.4) containing 3 mM ethylene glycol-bis(2-aminoethyl ether) tetraacetic acid (EGTA) were used to store the sperm at 4℃. The storage effect was evaluated using motility index and survival rate of sperm. Glucose and sodium citrate were found to be better diluents which maintained high motility and survival rate of sperm for a storage period of 10 days.
Some morphological changes of spermatozoa were observed during the cold storage with diluents. In particular, a detachment of the nuclear envelope and of the plasma membrane from the nucleus in spermatozoa was observed. Morphological normality of the stored spermatozoa diluted with 0.3 M glucose was better than that of the stored spermatozoa undiluted or diluted with Ca²+ free ASW containing 3 mM EGTA.
Various diluents of glucose, L. yokohamae serum, marine fish Ringer's solution, sodium citrate and Ca²+ free artificial seawater (ASW) (tris-HCl, pH 7.4) containing 3 mM ethylene glycol-bis(2-aminoethyl ether) tetraacetic acid (EGTA) were used to store the sperm at 4℃. The storage effect was evaluated using motility index and survival rate of sperm. Glucose and sodium citrate were found to be better diluents which maintained high motility and survival rate of sperm for a storage period of 10 days.
Some morphological changes of spermatozoa were observed during the cold storage with diluents. In particular, a detachment of the nuclear envelope and of the plasma membrane from the nucleus in spermatozoa was observed. Morphological normality of the stored spermatozoa diluted with 0.3 M glucose was better than that of the stored spermatozoa undiluted or diluted with Ca²+ free ASW containing 3 mM EGTA.
목차
Abstract
Introduction
Materials and Methods
Results
Discussion
Acknowledgements
References
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UCI(KEPA) : I410-ECN-0101-2009-529-015350024