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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The stf (Salmonella typhimurium fimbriae) operon consisting of stfACDEFG assumes to encode putative fimbriae. The complete stf operon is existed in S. typhimurium and S. choleraesuis, whereas it is absent in S. typhi. Analyses of the amino acid residues between major subunit StfA of the Stf fimbriae and those of known other fimbriaes suggested that Stf belongs to class I type fimbriae. Through comparison of StfD chaperone with the other fimbrial chaperones, and of C- terminus in subunits of Stf fimbriae, it belongs to FGS (with a short F1-G1 loop) subfamily. In order to investigate the expression of stf operon, we have constructed a Salmonella strain containing a chromosomal stfA::lacZYA transcriptional fusion, resulting in S. typhimurium χ8532. The strain χ8532 lacked the expression of β-galactosidase under normal culture conditions. However, with longer incubation time of the S. typhimurium χ8532, we have isolated 21 individual strains exhibiting Lac+ phenotype. Lac+ phenotype was appeared as approximately 0.03 frequency per generation. All isolates expressed lacZ constitutively in the various environmental conditions. Various global regulatory proteins including RpoS, OmpR, and CpxR were not involved in the regulation of the stf operon. A S. typhimurium χ8661 mutant lacking stfAC function attenuated 6.7 folds more than that of wild type χ3761 in the mouse virulence test, suggesting in the somehow involved in the Salmonella pathogenesis.
목차
Abstract
재료 및 방법
결과 및 고찰
요약
Acknowledgement
참고문헌
참고문헌 (30)
참고문헌 신청
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Bertani, G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62, 293-300.
Collinson, S. K., P. C. Doig, J. L. Doran, S. Clouthier, T. J. Trust, and W. W. Kay. 1993. Thin, aggregative fimbriae mediate binding of Salmonella enteritidis to fibronectin. J. Bacteriol. 175, 12-18.
Duguid, J. P., E. S. Anderson, and I. Campbell. 1966. Fimbriae and adhesive properties in Salmonellae. J. Pathol. Bacteriol. 92, 107-138.
Duguid, J. P., I. W. Smith, G. Dempster, and P. N. Edmunds. 1955. Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activity in Bacterium coli. J. Pathol. Bacteriol. 70, 335-348.
Bertani, G. 1951. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62, 293-300.
Collinson, S. K., P. C. Doig, J. L. Doran, S. Clouthier, T. J. Trust, and W. W. Kay. 1993. Thin, aggregative fimbriae mediate binding of Salmonella enteritidis to fibronectin. J. Bacteriol. 175, 12-18.
Duguid, J. P., E. S. Anderson, and I. Campbell. 1966. Fimbriae and adhesive properties in Salmonellae. J. Pathol. Bacteriol. 92, 107-138.
Duguid, J. P., I. W. Smith, G. Dempster, and P. N. Edmunds. 1955. Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activity in Bacterium coli. J. Pathol. Bacteriol. 70, 335-348.
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