무우 圃場에서 分離한 Rhizoctonia solani 5群 4型의 7菌株(AG 1-1A, AG 1-1B, AG 2-1, AG 2-2, AG 3, AG 4, AG 5)를 使用하여 ?度, pH, 窒素源, 炭素源이 菌의 生長에 미치는 影響을 알아보았다. 이들 菌絲融合群株別, 最適生長?度는 AG 2-1, AG 2-2, AG 4 등은 20℃에서 AG 1-1A, AG 1-1B, AG 3, AG 5 등은 25℃에서 生長이 가장 良好하였다. pH가 菌絲融合群別 生長에 미치는 影響은 共히 pH 6~7 範圍였다, R. solani의 各 菌株別 炭素源 利用度는 AG 1-1A, AG 1-IB, AG 2-2, AG 3, AG 5는 glucose, AG 2-1은 sucrose, AG4는 fructose가 좋았으나, glycerine, cellulose, lactose는 모든 菌株에서 生長이 不良하였다. 窒素源현이 菌絲融合群別 各 菌株의 生長에 미치는 影響은 Ca(NO₃)₂에서는 AG 1-1A, AG 1-IB, AG 4, asparagine에서는 AG 2-1, KNO₃에서는 AG 2-2, NaNO₃에서는 AG 5의 生長이 良好하였으나, NH₄NO₃ 첨가구에서는 共히 不良하였다. ?, 一般的으로 硝酸態窒素와 有機窒素는 암모니아態窒素보다 利用率이 높았다. C/N率이 各 群別 菌株의 生長에 마치는 影響은 C : N=1 : 1의 比率에서 生長이 가장 좋았고 炭素源보다는 窒素源이 生長에 많은 影響을 미쳤다.
The effects of temperature, pH, carbon and nitrogen sources on the growth of Rhizoctonia solani were studied by using five hyphal anastomosis groups(four cultural types, 7 isolates) of the fungus. The ranges of optimum temperature were 20℃ in the AG 2-1, AG 2-2 and AG 4, and 25℃ in the AG 1-1A, AG 1-1B, AG 3, AG 5. The optimum pH for the mycelial growth was 6-7 in the fungus. Glucose in the AG 1-1A, AG 1-1B, AG 2-2, AG 3 and AG 5, sucrose in the AG 2-1 and fructose in the AG 4 were the most effective for the mycelial growth, but glycerine, cellulose and lactose were not effectively utilized as nutrients. Ca(NO₃)₂ in the AG 1-1A, AG 1-IB and AG 4, asparagine in the AG 2-1, KNO₃ in the AG 2-2 and NaNO₃ in the AG 5 were the best nitrogen sources for the mycelial growth, but NH₄NO₃ was not easily utilized by the fungus. Nitrate and organic nitrogens for the fungal growth were utilized better than ammonium nitrogen.