우리나라 포도재배 지역인 포천, 남양주 및 수원의 포도나무 줄기혹병에 이병된 포장으로부터 채집한 줄기혹(gall), 수액(sap) 및 근권토양으로부터 56균주의 유사 Agrobacterium를 분리하였으며, 이들 중 7균주가 포도나무 유묘의 줄기에 혹을 형성시키는 병원성 Agrobacterium이었다. 병원성 Agrobacterium 균주는 Agrobacterium biovar의 선택배지에서의 생장, oxidase의 생성, Na L-tartrate로부터 알칼리의 성장, 2%NaCl 첨가 배지에서 생장, 3-ketolactose의 비생산, mesoerythritol과 ethanol로부터 산을 생성하지 못하는 등 Agrobacterium biovar 3인 A. vitis로 동정되었다.
Fifty-six bacterial strains were isolated from galls and sap of grapevine showing crown gall symptoms and rhizosphere soils of the plants with crown galls on stems in grapevine orchards in Namyangju, Pocheon and Suwon, Korea to examine the etiology of grapevine crown gall. Seven of the 56 strains were pathogenic bacteria which induced galls on grapevine seedlings. The 7 strains were classified as Agrobacterium biovar 3 on the basis of their growth on selective media and their cultural and biochemical properties, showing positive responses in oxidase production, growth in the presence of 2% NaCl and alkali production from Na L-tartrate, and negative responses in production of 3-ketolactose and acid production from meso-erythritol and ethanol. Also according to these results, all of the 7 pathogenic isolates were suggested to be Agrobacterium vitis.