목련흰비단병균 Sclerotium rolfsii의 균사생장 및 균핵형성에 대한 thianine, biotin, nicotinic acid, Pyridoxine, inositol과 DNA 및 RNA의 영향을 조사한 바 그 결과를 요약하연 다음과 같다. 1. 공시균은 thiamine결핍균으로서 균사생장 최적 thiamine 농도는 20γ/ℓ이고 이농도를 초과하면 오히려 균사생장이 억제되어 150γ/ℓ에서는 무첨가구와 거이 비슷한 균사생장을 하였다. 2. 공시균의 생장에 있어서 thiamine의 첨가에 따른 질소원 이용도는 NH₄NO₃>(NH₄)₂SO₄> asparagine> KNO₃의 순위이며 질소원별 thiamine 최적요구량은 KNO₃인 경우 12γ/ℓ, asparagine인 경우는 16γ/ℓ정도였다. 균핵형성량에 있어서는 KNO₃>NH₄NO₃>asparagine>(NH₄)₃SO₃의 순위로서 질소원별 thiamine 최 적량은 KNO₃, NNO₃, NH₄NO₃가 8γ/ℓ에서 균핵의 대부분이 형성되나 asparagine은 16γ/ℓ정도였다. 3. 배양액의 pH는 공시균이 생장을 개시하자마자 3.5 정도로 급격히 떨어지나 그 이후로는 생장량이 증가함에 따라 완만하게 떨어졌다. 그러나 pH 2.2 이하로는 내려가지 않았다. 4. Nicotinic acid는 공시균의 생장 및 균핵형성에 아무런 효과가 없었다. 그러나 thiamine 10γ/ℓ와의 공존하에서는 다소의 효과가 있는 것으로 nicotinic acid 7~10㎎/ℓ에서 가장 생장이 좋았고 그이상에서는 생육이 억제되었다. 5. Pyridoxine, biotin 및 inositol은 배지의 질소원이(NH₄)₂SO₄나 asparagine인 경우 모두 공시균의 균사생장 및 균핵형성에 아무런 효과가 없었다. 6. 공시균의 균사생장에 대한 각종 vitamin의 상호효과는 thiamine, biotin, Pyridoxine, inositol의 4가지 combination에 의해서도 thiamine이 첨가되지 않으면 거이 효과가 나타나지 않았다. 그러나 thiamine+pyridoxine, thiamine+inositol, thiamine+biotin+pyridoxine, thiamine+biotin+pyridoxine+inositol구에 있어서는 thiamine 단독첨가구와 동등 혹은 그 이상이지만 thiamine+biotin과 thiamine+biotin+inositol구는 오히려 떨어졌다. 균핵형성에 있어서는 thiamine단독구에 비하여 각구 모두 약간씩 증가 하였다. 7. 공시균의 균사생장에 대한 DNA와 RNA의 효과는 무첨가구에 비하여 다소 인정되는데 DNA보다는 RNA가 좀더 효과적 이었다. RNA는 2~6㎎/ℓ에서 DNA는 6㎎/ℓ에서 가장 좋았다. 균핵형성에 있어서는 효과가 없었다. 8. RNA의 농도가 증가함에 따라 또 thiamine의 농도가 증가함에 따라 균사생장량이 증가하는 것으로 thiamine의 존재하에 RNA의 효과가 뚜렷하게 나타났다. 그러나 균핵형성에는 효과가 없었다.
The study was performed to clear the effects of thiamine, biotin, nicotinic acid, pyridoxine, inositol, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) on the mycelial growth and the sclerotial production of Sclerotium rolfsii Sacc. isolated from Magnolia kobus. The results are abstracted as follows : 1. Tested fungus was thiamine-deficient and required thiamine 20γ/ℓ for maximum growth of mycelia. At higher concentrations than thiamine 20γ/ℓ, however, mycelial growth was decreased with increasing the concentrations and was inhibited little less than that of thiamine-free control at 150γ/ℓ. 2. The effecfivenesses of the nitrogen sources on the mycelial growth under the thiamine presence were recognized in order of NH₄NO₃>(NH₄)₂SO₄ >asparagine> KNO₃, and on the sclerotial production were KNO₃> NH₄NO₃>asparagine> (NH₄)₂SO₄. The optimum concentrations of thiamine were about 12γ/ℓ in KNO₃, about 16γ/ℓ in asparagine on the growth of mycelia, and were about 8γ/ℓ in KNO₃ and NH₄NO₃, l6γ/ℓ in asparagine on the production of sclerotia. 3. After the organism began to grow, the pH value of cultral filtrate was rapidly dropped down to about 3.5. Hereafter it was slowly fallen down as the growth amount was increased, but was not depreciated below pH 2.2. 4. Nicotinic acid was not effective individually on the mycelial growth and the sclerotial formation of tested fungus without thiamine, but slight effect of it was recognized with thiamine 10γ/ℓ, even though maximum growth was shown at 7-10㎎/ℓ. Beyond that concentration, however, mycelial growth was rather depressed. 5. When ammonium sulphate or asparagine as the nitrogen sources was used, pyridoxine, biotin and inositol had not any effectivenesses on the mycelial growth and the sclerotial production of examined fungus. 6. In the concentrations of thiamine, biotin, pyridoxine and inositol, as long as thiamine was not added in those, their correlating effects on the growth of the organism were not observed at all. Equivalent or more effects on the mycelial growth were recognized in combinations of thiamin +pyridoxine, thiamine+inositol, thiamine+biotin+pyridoxine, and thiamine+biotin +pyridoxine+inositol compared with thiamine alone, and in combinations of thiamine+biotin and thiamine+biotin+inositol, mycelial growth was inhibited rather than that of thiamine alone. Sclerotial production of those combinations was increased more than that of thiamine alone in dry weight. 7. The little effects of DNA and RNA on the mycelial growth of the organism were recognized compared with the control(DNA-and RNA-free), and RNA was more effective than DNA. Maximum growth of mycelia was observed at RNA 2-6㎎/ℓ and DNA 6㎎/ℓ. No effectivenesses on the sclerotial production were recognized in the RNA and DNA. 8. Mycelial growth of the organism was increased with increasing the concentrations of the RNA and the thiamine, that is, the effectiveness of RNA was revealed apparently under presence of thiamine, but was not shown in the sclerotial formation.