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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1999.12
- 수록면
- 313 - 318 (6page)
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Cloning of the 5' untranslated region (5' UTR) and Nterminus of the glycoprotein precursor (G2G1) open reading frame of tomato spotted wilt virus has been problematic, possibly because of the toxicity of a signal peptide at the beginning of the G2G1 protein precursor. The toxicity of the signal peptide to bacterial growth and the reason for the expression of the peptide gene in Escherichia coli were investigated by cloning the 5' UTR and the signal peptide sequence separately. Cells transformed with the plasmid containing both the first 30 amino acids of the glycoprotein and the 5' UTR showed a severe growth inhibition whereas transform ants harboring either the plasmid with the signal sequence or the 5' UTR alone did not show any inhibition. An E. coli promoter-like sequence was found in the 5' UTR and its promoter activity was confirmed with a promoter-less GUS gene cloned downstream of the 5' UTR. In the cloning of the Tomato spotted wilt virus (TSWV) glycoprotein G2G1 open reading frame all the recovered plasmids contained stop co dons in the signal sequence region. However, clones containing no stop codon were recovered when the signal sequence and the 5' UTR were cloned separately.
목차
Materials and Methods
Results
Discussion
Acknowledgements
References
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