인삼뿌리썩음病菌, Cylindrocarpon destructans(Zins.) Scholten을 接種한 人蔘切片으로 부터 얻은 纖維素分解酵素 및 펙틴質分解酵素의 力價는 그 濃度, 時間에 比例하였다. Cellulase (Cx), endo-polygalacturonase(endo-PG), endo-polymethylgalacturonase (endo-PMG), exo-polygalacturonase (exo-PG)와 exo-polymethylgalacturonase(exo-PMG)의 力價는 接種後 4日째 最大였으며 endo-PG와 endo-PMG는 1日 및 2日째는 全혀 檢出되지 않았으나 exo-PG와 exo-PMG는 檢出되었다. 接種後 6日째에는 모든 펙틴質分解酵素는 活性을 完全히 잃었으나 纖維素分解酵素는 높은 力價을 維持하였다. Cx, endo-PG, endo-PMG, exo-PG 및 exo-PMG의 最適 pH는 各各 6.0, 5.5, 8.0, 7.0~7.5, 8.5이였다. Cx, endo-PG, endo-PMG, 및 exo-PG의 最適 溫度는 各各 exo-PMG 66℃, 53℃, 41℃, 37℃, 40℃였다. 纖維素分解酵素는 共試한 16個 이온中에서 0.05M Hg??만이 抑制하였다. 펙틴質分解酵素는 이온에 따라 相異하였으나 0.005M Fe???과 0.05M Al???이 가장 顯著히 抑制하였다. 共試한 8個 農藥中에서 어느 것도 0.1% 有?成分으로는 모든 酵素作用을 抑制하지 못했으며 exo-PG만은 모든 農藥의 의하어 相當히 抑制되 었다. 그 中에서 Difolatan은 모든 펙틴質分解酵素을 가장 잘 抑制시켰다. 0.2M C??과 0.02M Fe???은 펙틴質分解酵素를 거의 抑制시켰으나 纖維素分解酵素는 같은 濃度에서 各各 30%, 70%에 이르렀다. Formalin은 exo-PG와 endo-PMG를 完全 抑制시켰으나 다른 酵素 특히 Cx는 그렇지 못했다. 0.8% Difolatan은 모든 酵素를 80% 以上 抑制하였으나 Cx는 그 以下였다. C. destructans가 分泌하는 纖維素分解酵素 및 펙틴質分解酵素는 人蔘뿌리썩음病과 密接한 關係를 가지고 있으며 이 病을 ?果的으로 防除하기 위하여 抑制되어야 하겠다.
The activities of pectolytic and cellulolytic enzymes produced from slices of ginseng root infected with Cylindrocarpon destructans(Zins.) Scholten were proportional to each concentration and reaction time. Activities of cellulase(Cx), endo-polygalacturonase(endo-PG), endo-polymethylgalacturonase(endo-PMG), exo-polygalacturonase(exo-PG), and exo-polymethylgalacturonase(exo-PMG) were maximum on the 4th day after inoculation. No endo-PG and endo-PMG were detected at the first and second days, while exo-PG exo-PMG were active. On the 6th day, all pectic enzymes were completely lost, whereas Cx remained at a high concentration. pH optima of Cx, endo-PG, endo-PMG, exo-PG, and exo-PMG were 6.0, 5.5, 8.0, 7.0 to 7.5, and 8.5, respectively. Temperature optima of Cx, endo-PG, endo-PMG, exo-PG, and exo-PMG were 66℃, 53℃, 41℃, 37℃, and 40℃, respectively. Cx was only inhibited by 0.05M Hg?? among 16 ions tested. Inhibitory effects of ions on pectolytic enzymes varied, however 0.005M Fe??? and 0.05M Al??? were the best in general. Among 8 fungicides, none of them inhibited all the enzymes studied at 0.1% active ingredients. Exo-PG were highly inhibited by all of the fungicides, of which difolatan was the most inhibitory to all the pectic enzymes. Ca?? at 0.2M and Fe??? at 0.02M completely inhibited all the pectolytic enzymes, and Cx was inhibited 30% and 70% at the same concentration, respectively. Formalin almost inhibited exo-PG and exo-PMG at 0.8% but not the other enzymes especially Cx. Difolatan at 0.8% inhibited all the enzymes concerned above 80%. The cellulolytic and pectolytic enzymes of C. destructans must be closely associated with the ginseng root rot and should be inhibited to control the disease effectively.
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