지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2002.6
- 수록면
- 109 - 114 (6page)
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초록· 키워드
오류제보하기
To evaluate the phylogenetic and taxonomic status of the phytoplasmas associated with water dropwort (Oenanthe javanica DC) disease in Korea and Japan, their 16S rDNA was analyzed. DNAs extracted from water dropworts collected in Korea (Kyongnam province) and Japan (Chiba prefecture) affected by witches’ broom and yellows were subjected to PCR using phytoplasmaspecific primers, which amplified a 1.4-kbp fragment that included the 16S rDNA. Phytoplasmas were characterized by RFLP analysis using AluⅠ, HaeⅢ, HhaⅠ, KpnⅠ, MseⅠ, and RsaⅠ restriction enzymes and by sequence analysis of the PCR products. The water dropwort witches’ broom (WDWB) and water dropwort yellows (WDY) 16S rDNA sequences were identical and closely related to onion yellows (OY, 99.9% identity), which belong to the aster yellows (AY) 16S-subgroup. However, the KpnⅠ RFLP analyses clearly distinguished the WDY and WDWB phytoplasmas from the OY phytoplasma. The phylogenetic analysis based on 16S rDNA showed that WDWB and WDY phytoplasmas are members of a relatively homogeneous group that evolved from a common ancestor.
목차
Materials and Methods
Results
Discussion
Acknowledgments
References
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UCI(KEPA) : I410-ECN-0101-2009-481-017565946