지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2005.9
- 수록면
- 283 - 287 (5page)
이용수
초록· 키워드
오류제보하기
Since the discovery of generation of O₂? in plant, many evidence for the oxidative burst (OXB) has been accumulated in various combinations of plant and pathogen or elicitor systems. O₂? generating system responsible for the OXB was coupled with oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) in microsomal fraction isolated from sliced aged potato tuber slices which were treated by hyphal wall components elicitor from Phytophthora infestans (HWC). We developed new assay method for quantitative measurement of oxygen radical O₂? by using electron spin resonance (ESR) analysis during elicitorinduced OXB on the surface of plant tissues. The ESR analysis using an O₂? trapper, Tiron (1, 2-dihydroxy-3, 5benzenedisulfonic acid), provided a convenient assay for detecting only O₂? during elicitor-induced OXB producing various active oxygen species (AOS) on plant tissue surface. Tiron was oxidized to Tiron semiquinon radical by O₂?, Quantity of the radical signal was measured by specific spectra on ESR spectroscopy. The level of O₂? was high in from surface of potato tuber tissue treated with hyphal cell wall elicitor (HWC) from Phytophthora infestans. There was no secondary OXB induction by H₂O₂ treatment in plant.
목차
Materials and methods
Results
Discussion
Acknowledgements
References
참고문헌 (0)
참고문헌 신청이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-481-017570747