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자료유형
학술저널
저자정보
저널정보
한국실험동물학회 Laboratory Animal Research Laboratory Animal Research Vol.21 No.2
발행연도
2005.6
수록면
110 - 115 (6page)

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The aim of the present study was to investigate the age-related onset of obesity in obese Zucker(fa/fa) rats as indicated by adipogenic biomarkers and genes. The Zucker rats aged 6, 8, 10, 12, and 16 wk were used to determine age-related changes in biochemical components including triglyceride, glucose, cholesterol, insulin and leptin in serum, and the expression of adipogenic genes such as acetyl CoA carboxylase (ACC) and peroxisome proliferator activated receptor y (PPARγ) in the epididymal fat pads. As a result, the serum levels of glucose and cholesterol did not significantly affected by ages. However, the level of triglyceride in serum resulted in a significant (p<0.05) increase after 8 wk of age and stayed at this level till 16 wk of age we examined. The level of insulin at 6 wk of age was significantly (p<0.05) lower than any other ages examined. Also, the Zucker rats aged 16 wk showed a markedly (p<0.05) higher serum leptin level when compared with the rats aged 6 and 8 wk. Especially, leptin level at 16 wk of age increased by a two-fold as compared with that at 6 wk old. We also observed age-related differences in the expressions of ACC and PPARy in the epididymal fats. In particular, ACC mRNA expression did show much of an age-associated alteration in fat tissues. The PPARγ expression in rats aged 6 wk was to be much lower (p<0.05) compared with the rats aged 8, 10, 12 and 16 wk. From the evidences we observed here, it is suggested that preobese status at 6 wkold Zucker(fa/fa) rats rapidly developed obesity around at 8 wk of age as indicated by biomarkers and consequently showed pathophysiological symptom of obesity after 8 wk of age.

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