메뉴 건너뛰기
Library Notice
Institutional Access
If you certify, you can access the articles for free.
Check out your institutions.
ex)Hankuk University, Nuri Motors
Log in Register Help KOR
Subject
Humanities
General Humanities History Philosophy Religious Studies Linguistics and Literature Other Humanities
Social science
General Social Science Business Management Tourism Education Military Science Library and Information Science Law Sociology Journalism and Media Studies Psychological Science Political Science and Diplomacy Geography Public Administration Economics International Trade Anthropology Other Social Science
Natural science
General Natural Science Physics Biology Life Sciences Mathematics/Statistics Astronomy Earth Science Chemistry Other Natural Science
Engineering
General Engineering Architectural Engineering Mechanical Engineering Industrial Engineering Material/Energy Engineering Electrical and Electronic Engineering Marine and Ocean Engineering Chemical Engineering Computer Science Other Engineering
Medical science
General Medical Science Nursing Science Veterinary Pharmacy Traditional Korean Medicine Basic Medicine Clinical Medicine Other Medicine
Agriculture and Fishery
General Agriculture and Fishery Agriculture Fishery Science Food Science Forestry Animal Husbandry Marine Transportation Studies Other Agriculture and Fishery
Art and Kinesiology
General Arts Design Fine Arts Cosmetology Photography Music Fashion Kinesiology Performing Media Arts Other Arts and Kinesiology
Interdisciplinary Studies
Interdisciplinary Research Science and Technology Studies Brain and Cognitive Science Technology Policy Other Interdisciplinary Studies

KOR

Conditioned Medium of Soybean Extract Treated Osteoblasts Inhibits RANKL Induced Differentiation of Osteoclasts
Recommendations
Search
Questions

대두추출물을 처리한 조골세포 조건배양액은 RANKL에 의해 유도된 파골세포 분화를 억제

논문 기본 정보

Type
Academic journal
Author
Journal
The korean Society of Food Science and Nutrition Journal of the Korean Society of Food Science and Nutrition Vol.39 No.1 KCI Accredited Journals SCOPUS
Published
2010.1
Pages
64 - 70 (7page)

Usage

cover
📌
Topic
📖
Background
🔬
Method
🏆
Result
Conditioned Medium of Soybean Extract Treated Osteoblasts Inhibits RANKL Induced Differentiation of Osteoclasts
Ask AI
Recommendations
Search
Questions

Abstract· Keywords

Report Errors
Soybean is of particular interest as a food supplement of isoflavones for inhibiting bone resorption in postmenopausal woman. These beneficial effects of isoflavones are caused by functioning as partial agonists or antagonists of estrogen, of which anti-resorptive effect is mediated indirectly through paracrine factors produced by osteoblasts that act on osteoclasts. In this study, the indirect effect of soybean on osteoclastic differentiation of RAW264.7 cells were investigated. The conditioned medium was collected from MC3T3-E1 osbeoblasts treated with 0.001 ㎎/mL~0.1 ㎎/mL soybean extracts for 6 days, mixed in 1:1 ratio with osteoclast medium, and then added into RAW264.7 cells with receptor activator of nuclear factor kappa B ligand (RANKL), a differentiation inducer for 3 days. Of paracrine factors in the conditioned medium, the protein expression of osteoprotegerin (OPG) with soybean extract was specifically higher in a dose dependent manner than with 10?? M~10?? M of estrogen, genistein or daidzein standards. In RAW264.7 cells, the conditioned medium with soybean inhibited RANKL induced osteoclastic differentiation as total number of multinucleated tartrateresistant alkaline phosphatase (TRAP)-positive osteoclasts and protein expression of MMP-9 were significantly decreased. Coupled with the low expression of estrogen receptor α and β proteins in RANKL treated RAW264.7 cells, we demonstrate that the conditioned medium of soybean treated osteoblasts inhibits RANKL induced differentiation of osteoclasts with the selective expression of OPG in osteoblasts.
#soybean extract #conditioned medium of osteoblasts #RANKL #OPG #osteoclastic differentiation

Contents

Abstract
서론
재료 및 방법
결과 및 고찰
요약
감사의 글
문헌

References (28)

Add References
1. [학술지(정기간행물)] - Parfitt AM / 1994 / Osteonal and hemi-osteonal remodeling: The spatial and temporal framework for signal traffic in adult human bone / J Cell Biochem 55 : 273 ~ 286 google schola 2. [학술대회논문] - Udagawa N / 1990 / Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells / Proc Natl Acad Sci USA : 7260 ~ 7264 google schola 3. [학술지(정기간행물)] - Kong YY / 2001 / Osteoprotegerin and receptor activator of nuclear factor κB ligand in the pathogenesis and treatment of rheumatoid arthritis / Arthritis Rheum 44 : 253 ~ 259 google schola 4. [학술지(정기간행물)] - Milsicek RJ / 1994 / Interaction of naturally occurring nonsteroidal estrogen with expressed recombinant human estrogen receptor / J Steroid Biochem Mol Biol 49 : 153 ~ 160 google schola 5. [학술지(정기간행물)] - Kurolouchi K / 1998 / TNF-α increases expression of IL-6 and ICAM-1 genes through activation of NK-κB in osteoblast-like ROS17/2.8 cells / J Bone Miner Res 13 : 1290 ~ 1299 google schola

Recommendations

It is an article recommended by DBpia according to the article similarity. Check out the related articles!

Related Authors

Frequently Viewed Together

Effects of Petasites japonicus and Momordica charantia L. Extracts on MC3T3-E1 Osteoblastic Cells
Suk-Hee Ji ,  Do-Hwan Ahn ,  Mi Journal of the Korean Society of Food Science and Nutrition 2010 .02
Inhibition Effects of Natural Products on Osteoclast Differentiation
Hyo Jung Lee ,  Mi Hee Yu ,  Syng-Ook Lee and others 2 Korean journal of food science and technology 2005 .12
Effects of Asparagus cochinchinensis (Lour.) Merr. on the Stimulation ofOsteoblast Differentiation and Inhibition of Osteoclast Generation
Seung Youn Lee ,  Si Na Kim ,  Jong Keun Kim Journal of the Korean Society of Food Science and Nutrition 2008 .01
The Effects of Baicalein on Osteoclast Differentiation from Bone Marrow Derived Macrophage
Ji Kwang Yun ,  Yoon-Hee Cheon ,  Ju-Young Kim and others 7 Anatomy & Biological Anthropology 2014 .06
NF-κB ANF-κB Activation and Cyclooxygenase-2 Expression Induced by Toll-Like Receptor Agonists can be Suppressed by Isoliquiritigenin
Se-Jeong Pa ,  k ,  Seung-Ju Yang Korean journal of food science and technology 2009 .04

Recently viewed articles

Landslide prediction and detection application using low-power Wireless Sensor Network
Jae Yeon Park, Ki Sung Lee, Jin Ho Kim 외 1명The HCI Society of Korea2017.02
 보수의 새로운 길 모색
송복Hansun Foundation2017.09
 항공영화〈R2B：리턴투베이스〉의 BPL엔터테인먼트 이미지 구축연구
강익모Korean Society For Journalism And Communication Studies2012.10

Comments(0)

0

Write first comments.

UCI(KEPA) : I410-ECN-0101-2010-511-002501150