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논문 기본 정보

자료유형
학술저널
저자정보
Young-Eun Cho (안동대학교) Ria-Ann R. Lomeda (안동대학교) Sang-Hoon Ryu (안동대학교) Jong-Hwa Lee (안동대학교) John H. Beattie (Rowett Research Institute) In-Sook Kwun (안동대학교)
저널정보
한국영양학회 Nutrition Research and Practice Nutrition Research and Practice Vol.1 No.1
발행연도
2007.3
수록면
29 - 35 (7page)

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이 논문의 연구 히스토리 (16)

2008

[OP-18] Zinc regulation of Runx2 expression through BMP-2 signaling in osteoblastic MC3T3-E1 cells
Young-Eun Cho ,  Hong-In Shin ,  In-Sook Kwun 한국식품영양과학회 학술대회발표집 2008.10 학술대회자료
[SP2-1] Zinc Modulation of Leptin Signaling in Osteoblastic Mc3T3-E1 Cells
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Md. Jahangi 외 7명 한국식품영양과학회 학술대회발표집 2008.10 학술대회자료

2007

Zinc deficiency negatively affects alkaline phosphatase and the concentration of Ca, Mg and P in rats
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Sang-Hoon Ryu 외 4명 Nutrition Research and Practice 2007.06 학술저널
Zinc deficiency negatively affects alkaline phosphatase and the concentration of Ca, Mg and P in rats
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Sang-Hoon Ryu 외 4명 Nutrition Research and Practice 2007.06 학술저널
Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3 E1 cells
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Sang-Hoon Ryu 외 3명 Nutrition Research and Practice 2007.03 학술저널
Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3 E1 cells
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Sang-Hoon Ryu 외 3명 Nutrition Research and Practice 2007.03 학술저널

2006

Bioactive Polyglycolic Acid (PGA) or Polylactic Acid (PLA) Polymers on Extracellular Matrix Mineralization in Osteoblast-like Mc3T3-E1 Cells
Young-Eun Cho ,  Hye-Jin Kim ,  Yong-Ha Kim 외 6명 Nutritional Sciences 2006.11 학술저널
[P10-281] Zn deficiency delays bone-marker gene and bone-specific transcription factor runx2 expression in osteoblastic MC3T3-E1 cells
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Hong-In Shin 외 2명 한국식품영양과학회 학술대회발표집 2006.10 학술대회자료
[P10-280] Zn deficiency increases leptin and leptin receptor (OB-Rb) gene and protein expression which may activates JAK-STAT signalling pathway in osteoblastic MC3T3-E1 cells
Young-Eun Cho ,  Ria-Ann R. Lαneda ,  Hong-In Shin 외 1명 한국식품영양과학회 학술대회발표집 2006.10 학술대회자료
[P10-279] Ethanol extracts of the roots of Glycyrrhiza uralensis Fischincrease the cell prolileration and bone marker enzyme alkaline phosphatase activity in osteoblastic MC3T3-E1 cells
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  In-Sook Kwun 한국식품영양과학회 학술대회발표집 2006.10 학술대회자료
[OP-07] Leptin Signaling through JAK-STAT Pathway under Zn Deficiency in Osteoblastic MC3T3-E1 Cells
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Bong-Hyuk Choi 외 3명 한국식품영양과학회 학술대회발표집 2006.10 학술대회자료

2005

Zinc Deficiency Decreased Alkaline Phosphatase Expression and Bone Matrix Ca Deposits in Osteoblast-like MC3T3-E1 Cells
Young-Eun Cho ,  Ria-Ann R. Lomeda ,  Yang-Ha Kim 외 5명 Nutritional Sciences 2005.11 학술저널
[P10-195] Beneficial Effect of Biodegradable Polyglycolide (PGA) or Polylactide (PLA) Polymers on Osteoblastic MC3T3-E1 Cell Morphology
Young-Eun Cho ,  Hye-Jin Kim ,  Ria-Ann R. Lomeda 외 7명 한국식품영양과학회 학술대회발표집 2005.10 학술대회자료
[P10-194] Zn increased Ca deposit and alkaline phosphatase in extracellular matrix and also affects bone-related gene transcription factor Run×2 and leptin-receptor gene expression
Young-Eun Cho ,  Hye-Jin Kim ,  Sook-Ja Kim 외 5명 한국식품영양과학회 학술대회발표집 2005.10 학술대회자료
[P10-158] Red Yeast Rice Extracts Increase the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells
Young-Eun Cho ,  Sook-Ja Kim ,  Hyun-Jung Kim 외 6명 한국식품영양과학회 학술대회발표집 2005.10 학술대회자료
[S4-5] Extracellular Matrix Mineralization and Bone-related Gene Expression in Osteoblastic MC3T3-E1 Cells under Zinc Deficiency
Young-Eun Cho ,  Sook-Ja Kim ,  Hyun-Jung Kim 외 4명 한국식품영양과학회 학술대회발표집 2005.10 학술대회자료

초록· 키워드

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Trace mineral studies involving metal ion chelators have been conducted in investigating the response of gene and protein expressions of certain cell lines but a few had really focused on how these metal ion chelators could affect the availability of important trace minerals such as Zn, Mn, Fe and Cu. The aim of the present study was to investigate the availability of Zn for the treatment of MC3T3-E1 osteoblast-like cells and the availability of some trace minerals in the cell culture media components after using chelexing resin in the FBS and the addition of N,N,N’,N’-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN, membrane-permeable chelator) and diethylenetriaminepentaacetic acid (DTPA, membrane-impermeable chelator) in the treatment medium. Components for the preparation of cell culture medium and Zn-treated medium have been tested for Zn, Mn, Fe and Cu contents by atomic absorption spectrophotometer or inductively coupled plasma spectrophotometer. Also, the expression of bone-related genes (ALP, Runx2, PTH-R, ProCOL I, OPN and OC) was measured on the cellular Zn depletion such as chelexing or TPEN treatment. Results have shown that using the chelexing resin in FBS would significantly decrease the available Zn (p＜0.05) (39.4 ± 1.5 μM vs 0.61 ± 10.15 μM) and Mn (p＜0.05) (0.74 ± 0.01 μM vs 0.12 ± 0.04 μM). However, levels of Fe and Cu in FBS were not changed by chelexing FBS. The use of TPEN and DTPA as Zn-chelators did not show significant difference on the final concentration of Zn in the treatment medium (0, 3, 6, 9, 12 μM) except for in the addition of higher 15 μM ZnCl₂ which showed a significant increase of Zn level in DTPA-chelated treatment medium. Results have shown that both chelators gave the same pattern for the expression of the five bone-related genes between Znand Zn+, and TPEN-treated experiments, compared to chelex-treated experiment, showed lower bone-related gene expression, which may imply that TPEN would be a stronger chelator than chelex resin. This study showed that TPEN would be a stronger chelator compared to DTPA or chelex resin and TPEN and chelex resin exerted cellular zinc depletion to be enough for cell study for Zn depletion.
#Zn depletion #metal ion chelators #DTPA #TPEN #chelexing #MC3T3-E1 cells

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Abstract
Introduction
Materials and Methods
Results
Discussion
Literature cited

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