The purpose of this study was to examine that the effect of Peony-Licorice (PL) mixture supplementation on post-exercise blood glucose and lactate in college basketball players. Twelve college male basketball players were participated in this study. The subjects participated in two trials, water supplementation and PL supplementation. They did 20-meter shuttle run test, and measured blood glucose and lactate level before, immediately, after 3 minutes, and after 5 minutes the test. There was an interaction effect on blood glucose and lactate levels (p<0.05). Blood glucose and lactate levels were decreased in PL group after the test. In conclusion, PL supplementation could affect changing blood glucose level and decreasing lactate level. Therefore, we could speculate that PL supplementation would be positive ergogenic acid for improving exercise performance in college male basketball players.