지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 71 - 76 (6page)
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초록· 키워드
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We demonstrate that baicalein, a bioactive flavonoid originally isolated from Scutellaria baicalensis, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of peritoneal macrophages and RAW 264.7 cells with baicalein inhibited LPS-stimulated nitric oxide production in a dose-related manner. Immunohistochemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression in RAW 264.7 cells. Immunostaining of p65, EMSA, and reporter gene assay showed that baicalein inhibited NF-κB nuclear translocation, DNA binding, and transcriptional activation, respectively. Collectively, these series of experiments indicate that baicalein inhibits iNOS gene expression by blocking NF-κB nuclear translocation. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of baicalein on iNOS suggest that baicalein may represent a useful anti-inflammatory agent.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
참고문헌 (19)
참고문헌 신청
Aldridge, C. / 2008 / Lipopolysaccharide-stimulated RAW 264.7 macrophageinducible nitric oxide synthase and nitric oxide productionis decreased by an omega-3 fatty acid lipid emulsion / J.Surg. Res 149:296~302
Chen, C.J. / 2004 / Inhibitionof inducible nitric oxide synthase expression by baicaleinin endotoxin/cytokine-stimulated microglia / Biochem. Pharmacol 67:957~965
Chomczynski, P / 1995 / Substitution of chloroformby bromo-chloropropane in the single-step method ofRNA isolation / Anal. Biochem 225:163~164
Dunnett,C.W / 1955 / A multiple comparison procedure for comparingseveral treatments with a control / J. Am. Statistics.Assoc 50:1096~1121
Green, L.C. / 1982 / Analysis of nitrate,nitrite, and [15N]nitrate in biological fluids / Anal. Biochem 126:131~138
Chen, C.J. / 2004 / Inhibitionof inducible nitric oxide synthase expression by baicaleinin endotoxin/cytokine-stimulated microglia / Biochem. Pharmacol 67:957~965
Chomczynski, P / 1995 / Substitution of chloroformby bromo-chloropropane in the single-step method ofRNA isolation / Anal. Biochem 225:163~164
Dunnett,C.W / 1955 / A multiple comparison procedure for comparingseveral treatments with a control / J. Am. Statistics.Assoc 50:1096~1121
Green, L.C. / 1982 / Analysis of nitrate,nitrite, and [15N]nitrate in biological fluids / Anal. Biochem 126:131~138
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UCI(KEPA) : I410-ECN-0101-2013-513-000636845