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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 167 - 171 (5page)
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초록· 키워드
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α-synuclein (α-syn) is implicated in the pathogenesis of Parkinson's disease (PD) and other related diseases. We have previously reported that α-syn binds to the cell membranes in a transient and reversible manner. However, little is known about the physiologic function and/or consequence of this association. Here, we examined whether chemically induced perturbations to the cellular membranes enhance the binding of α-syn, based on hypothesis that α-syn may play a role in maintenance of membrane integrity or repair. We induced membrane perturbations or alterations in α-syn-overexpressing human neuroblastoma cells (SH-SY5Y) by treating the cells with hydrogen peroxide (H₂O₂) or oleic acid. In addition, membranes fractionated from these cells were perturbed by treating them with proteinase K or chloroform. Dynamic interaction of α-syn to the membranes was analyzed by the chemical cross-linking assay that we developed in the previous study. We found that membrane interaction of α-syn was increased upon treatment with membrane-perturbing reagents in a dose and time dependent manner. These results suggest that perturbations in the cellular membranes cause increased binding of α-syn, and this may have significant implication in the physiological function of α-syn in cells.
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참고문헌 (14)
참고문헌 신청
1. [학술지(정기간행물)] - Athanassiadou A / 1997 / Mutation in the alpha-synuclein gene identified in families with parkinson's disease / Science 276 : 2045 ~ 2047
2. [학술지(정기간행물)] - Burre J / 2010 / Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro / Science 329 : 1663 ~ 1667
3. [학술지(정기간행물)] - Castagnet PI / 2005 / Fatty acid incorporation is decreased in astrocytes cultured from alpha-synuclein gene-ablated mice / J Neurochem 94 : 839 ~ 849
4. [학술지(정기간행물)] - Darios F / 2010 / Alpha-synuclein sequesters arachidonic acid to modulate SNARE-mediated exocytosis / EMBO Rep 11 : 528 ~ 533
5. [학술지(정기간행물)] - Davidson WS / 1998 / Stabilization of alpha-synuclein secondary structure upon binding to synthetic membranes / J Biol Chem 273 : 9443 ~ 9449
2. [학술지(정기간행물)] - Burre J / 2010 / Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro / Science 329 : 1663 ~ 1667
3. [학술지(정기간행물)] - Castagnet PI / 2005 / Fatty acid incorporation is decreased in astrocytes cultured from alpha-synuclein gene-ablated mice / J Neurochem 94 : 839 ~ 849
4. [학술지(정기간행물)] - Darios F / 2010 / Alpha-synuclein sequesters arachidonic acid to modulate SNARE-mediated exocytosis / EMBO Rep 11 : 528 ~ 533
5. [학술지(정기간행물)] - Davidson WS / 1998 / Stabilization of alpha-synuclein secondary structure upon binding to synthetic membranes / J Biol Chem 273 : 9443 ~ 9449
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UCI(KEPA) : I410-ECN-0101-2013-510-000412808