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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 227 - 233 (7page)
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This study aimed at developing cryopreservation protocol for chrysanthemum (Dendranthema grandiflora Tzelevcv. peak) shoot apices based on droplet-vitrification procedure, which is a combination of droplet-freezing and solution based vitrification. Progressive preculture of shoot apices in liquid MS medium supplemented with 0.3 and 0.7 M sucrose for 31 and 17 hours, respectively, was found optimum among preculture treatments tested. The composition of both loading and vitrification solutions significantly affected recovery growth of shoot tips before and after cryopreservation. Balancing glycerol and sucrose concentrations in the solutions was beneficial for recovery growth. The highest recovery after cryopreservation was observed when apical shoot tips were extracted from 4-week-old in vitro plantlets, progressively precultured with 0.3-0.5-0.7 M sucrose for 32-16-6 hours, respectively, then treated with loading solution comprising of 1.9 M glycerol + 0.5 M sucrose (35% PVS3 solution). Apices were then dehydrated with the vitrification solution consisted of 50% glycerol + 50% sucrose for 90 minutes then directly immersed in liquid nitrogen.
목차
Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과 및 고찰
Ⅳ. 결론
감사의 글
참고문헌
참고문헌 (20)
참고문헌 신청
1. [학술지(정기간행물)] - Ahn YH / 1994 / Survival by vitrification of Dendranthema graniflorum L. shoot tips for cryopreservtion / Journal of the Korean Society for Horticultural Science 35 : 499 ~ 506
2. [단행본] - Engelmann F / 2000 / Importance of cryopreservation for the conservation of plant genetic resources in Cryopreservation of Tropical Germplasm - Current Research Progress and Application / JIRCAS, Tsukuba & IPGRI, Rome : 8 ~ 20
3. [학술지(정기간행물)] - Fahy GM / 1984 / Vitrification as an approach to cryopreservation / Cryobiology 21 : 407 ~ 426
4. [학술지(정기간행물)] - Fahy GM / 1990 / Cryoprotectant toxicity and cryoprotectant toxicity reduction: in search of molecular mechanisms / Cryobiology 27 : 247 ~ 268
5. [학술지(정기간행물)] - Fahy GM / 2004 / Improved vitr ification solutions based on the predictability of vitrification solution toxicity / Cryobiology 48 : 22 ~ 35
2. [단행본] - Engelmann F / 2000 / Importance of cryopreservation for the conservation of plant genetic resources in Cryopreservation of Tropical Germplasm - Current Research Progress and Application / JIRCAS, Tsukuba & IPGRI, Rome : 8 ~ 20
3. [학술지(정기간행물)] - Fahy GM / 1984 / Vitrification as an approach to cryopreservation / Cryobiology 21 : 407 ~ 426
4. [학술지(정기간행물)] - Fahy GM / 1990 / Cryoprotectant toxicity and cryoprotectant toxicity reduction: in search of molecular mechanisms / Cryobiology 27 : 247 ~ 268
5. [학술지(정기간행물)] - Fahy GM / 2004 / Improved vitr ification solutions based on the predictability of vitrification solution toxicity / Cryobiology 48 : 22 ~ 35
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