지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 269 - 275 (7page)
이용수
초록· 키워드
오류제보하기
The objective of this study was to develop a rapid and reliable PCR method for sexing of morula or blastocyst stage bovine embryo. BOV97M and bovine 1.715 satellite DNA sequences were selected for amplification of male and bovine specific DNA, respectively. But the unbalanced number of copies of these two repetitive sequences required some modification of PCR method. Karyotyping of blastomeres were carried for the confirmation of sex determination in bovine embryos. The coincidence rate of sex between biopsied-single blastomere and matched blastocyst was 80.0%. When in vivo- and in vitro- derived embryos were compared, 61.8% and 56.7% were male in in vitro- and in vivo-derived embryos, respectively. In vivo-derived embryos showed better hatching rate than in vitro-derived embryos following biopsy of blastomeres. In conclusion, rapid and effective PCR could be applied to sexing of bovine preimplantation embryos using single blastomere. The sensitivity of this assay may eliminate the need for biopsy of more than one nucleated blastomere and reduce trauma to the embryos derived from biopsy procedure.
목차
Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅳ. 결과 및 고찰
Ⅳ. 결론
감사의 글
참고문헌
참고문헌 (27)
참고문헌 신청
1. [학술지(정기간행물)] - Avery B / 1992 / Morphological development and sex of bovine in vitrofertilized embryos / Mol. Reprod. Dev 32 : 265 ~ 270
2. [단행본] - Bruce A / 1994 / Molecular biology of the cell. 3rd ed / Garland Publishing, Inc : 863 ~ 910
3. [학술지(정기간행물)] - Carvalho RV / 1996 / Survival rates and sex ratio of bovine IVF embryos frozen at different developmental stages on Day 7 / Theriogenology 45 : 489 ~ 498
4. [학술지(정기간행물)] - Carvalho RV / 1995 / Effect of stage of development on sex ratio and survival after freezing of Day 7 bovine IVF embryos / Theriogenology 43 : 183 ~
5. [학술지(정기간행물)] - Chen CM / 1999 / Gender determination in single bovine blastomeres by polymerase chain reaction amplification of sex-specific polymorphic fragments in the amelogenin gene / Mol. Reprod. Dev 54 : 209 ~ 214
2. [단행본] - Bruce A / 1994 / Molecular biology of the cell. 3rd ed / Garland Publishing, Inc : 863 ~ 910
3. [학술지(정기간행물)] - Carvalho RV / 1996 / Survival rates and sex ratio of bovine IVF embryos frozen at different developmental stages on Day 7 / Theriogenology 45 : 489 ~ 498
4. [학술지(정기간행물)] - Carvalho RV / 1995 / Effect of stage of development on sex ratio and survival after freezing of Day 7 bovine IVF embryos / Theriogenology 43 : 183 ~
5. [학술지(정기간행물)] - Chen CM / 1999 / Gender determination in single bovine blastomeres by polymerase chain reaction amplification of sex-specific polymorphic fragments in the amelogenin gene / Mol. Reprod. Dev 54 : 209 ~ 214
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UCI(KEPA) : I410-ECN-0101-2013-480-000275182