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The purpose of this study was to investigate the acute influences of cardiovascular, respiratory, and immune system on 60min-prolonged exercise test of HR at 5O%VO₂max intensity in an exposed normobaric hypoxic environment. The subjects were 15 male college students(19.1±1.0yr, BMI 22.3±2.1, fat mass 10.9±3.1㎏, %fat 15.5±3.2%, LBM 55.3±5.8㎏, VO₂max 39.0±3.3㎖/㎏/min). Items of measurement after 60min-prolonged exercise on sea level and 3000m altitude were HR, SV, CO, SVR, SBP, DBP, MAP, TVC of cardiovascular system, VO₂, VO₂max, RR, RQ, VE, Mets, VT, VCO₂ of respiratory system, and ACTH, HGH, cortisol, epinephrine, norepinephrine, RBC, WBC, HCT, Hb, NKcell, Tcell, Bcell et al of immune system and hormone. As the result of the verifying hypothesis, the results may suggest new findings: first, VO₂, RR, VE, in respiratory system increased significantly at 3000m altitude better than sea level(p<.01). Second, HR, SV, CO, SVR in respiratory system increased significantly at 3000m altitude better than sea level(p<.01). Third, Cortisol, norepinephrine, WBC, NKcell in immune system and hormone increased significantly at 3000m altitude better than sea level(p<.01). In conclusion, 60min-prolonged exercise on normobaric hypoxic environment may affect cardiovascular, respiratory, and immune system.