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논문 기본 정보

자료유형
학술저널
저자정보
Margaret T. Mmbaga (Tennessee State University) Ainong Shi (Syngenta Seeds) Mee-Sook Kim (국민대학교)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.27 No.2
발행연도
2011.6
수록면
120 - 127 (8page)

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초록· 키워드

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While many isolates of Alternaria alternata are common saprophytes on trees and shrubs, this study clearly demonstrated that A. alternata is a primary pathogen in lilac (Syringa sp.), causing a leaf-blight that affects different Syringa species. Isolates of Alternaria sp. were collected from leaf blight samples of lilacs in the field. The internal transcribed spacer (ITS) region and morphological characterization were used to identify lilac blight pathogen. Based on 100% ITS nucleotide sequence identities to the Alternaria genus in the GenBank and morphological features, these isolates were identified as A. alternata. Disease symptoms were reproduced in lilac plants inoculated with A. alternata mycelial plugs and sprayed with a fungus-free culture filtrate, indicating that pathogenesis in lilac involves secondary metabolites or toxins. Diagnostic primers were developed to detect Alternaria sp. and A. alternata in lilac leaf blight based on ITS region and four known genes associated with pathogenesis in A. alternata: mixed-linked glucanase precursor, endopolygalacturonase, hsp70, and histone genes. The results from our study indicated A. alternata is a primary pathogen in lilac leaf blight, and these diagnostic primers can be used as a tool for the fast detection of A. alternata associated with lilac leaf blight.

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Materials and Methods
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UCI(KEPA) : I410-ECN-0101-2013-481-000712567