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논문 기본 정보

자료유형
학술저널
저자정보
Hyo Jun Park (고려대학교) Chang Seob Kwon (한국과학기술원) Joo-Yong Woo (고려대학교) Gil-Je Lee (고려대학교) Young Jin Kim (고려대학교) Kyung-Hee Paek (고려대학교)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.27 No.2
발행연도
2011.6
수록면
170 - 182 (13page)

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초록· 키워드

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Plants possess multiple resistance mechanisms that protect themselves against pathogen attack. To identify unknown components of the defense machinery in Arabidopsis, gene-expression changes were monitored in Arabidopsis thaliana under 18 different biotic or abiotic conditions using a DNA microarray representing approximately 25% of all Arabidopsis thaliana genes (www.genevestigator.com). Seventeen genes which are early responsive to salicylic acid (SA) treatment as well as pathogen infection were selected and their T-DNA insertion mutants were obtained from SALK institute. To elucidate the role of each gene in defense response, bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 was inoculated onto individual T-DNA insertion mutants. Four mutants exhibited decreased resistance and five mutants displayed significantly enhanced resistance against Pst DC3000-infection as measured by change in symptom development as compared to wild-type plants. Among them, member of uridin diphosphate (UDP)-glycosyltransferase (UGT) was of particular interest, since a UGT mutant (At1g05680) showed enhanced resistance to Pst-infection in Arabidopsis. In systemic acquired resistance (SAR) assay, this mutant showed enhanced activation of SAR. Also, the enhanced SAR correlated with increased expression of defenserelated gene, AtPR1. These results emphasize that the glycosylation of UGT74E2 is a part of the SA-mediated disease-resistance mechanism.

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Materials and Methods
Results
Discussion
Acknowledgments
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UCI(KEPA) : I410-ECN-0101-2013-481-000712623