지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 185 - 190 (6page)
이용수
초록· 키워드
오류제보하기
Adenovirus type 5 (Ad5) vectors have been used for gene transfer to a wide variety of cell types in vivo and in vitro. The advantages of adenovirus vectors include the high titer of virus readily obtained in large scale preparations, their ability to transduce dividing and non dividing cells, and the high level of transgene expression. Since adenovirus vectors do not integrate in host cell DNA, there is a lack of insertional mutagenesis. However, many human tumor cells lack expression of the adenovirus 5 receptors and contain areas of hypoxia. In order to identify the pattern of replication and gene expression of oncolytic adenovirus in hypoxic condition, multiple different fiber modified Ads (Ad5F/S11, Ad5F/S35, Ad5F/K7, Ad5F/K21, and Ad5F/RGD) was compared. The replication of all fiber modified adenovirus was inhibited in hypoxic condition in HEK 293 cells, but gene expression has variety on different tumor cell lines and the level of coxackievirus and adenovirus receptor (CAR) expression. These data suggest that CAR expression pattern and hypoxic condition of tumor are considered for optimal oncolytic adenovirus application.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (23)
참고문헌 신청
Blackwell JL / 1999 / Retargeting to EGFR enhances adenovirus infection efficiency of squamous cell carcinoma / Arch Otolaryngol Head Neck Surg 125:856~863
Brough DE / 1996 / A gene transfer vector-cell line system for complete functional complementation of adenovirus early regions E1 and E4 / J Virol 70:6497~6501
Cho WK / 2004 / Oncolytic effects of adenovirus mutant capable of replicating in hypoxic and normoxic regions of solid tumor / Molecular therapy: the journal of the American Society of Gene Therapy 10:938~949
Cripe TP / 2001 / Fiber knob modifications overcome low, heterogeneous expression of the coxsackievirus-adenovirus receptor that limits adenovirus gene transfer and oncolysis for human rhabdomyosarcoma cells / Cancer Res 61:2953~2960
Flomenberg PR / 1987 / Molecular epidemiology of adenovirus type 35 infections in immunocompromised hosts / J Infect Dis 155:1127~1134
Brough DE / 1996 / A gene transfer vector-cell line system for complete functional complementation of adenovirus early regions E1 and E4 / J Virol 70:6497~6501
Cho WK / 2004 / Oncolytic effects of adenovirus mutant capable of replicating in hypoxic and normoxic regions of solid tumor / Molecular therapy: the journal of the American Society of Gene Therapy 10:938~949
Cripe TP / 2001 / Fiber knob modifications overcome low, heterogeneous expression of the coxsackievirus-adenovirus receptor that limits adenovirus gene transfer and oncolysis for human rhabdomyosarcoma cells / Cancer Res 61:2953~2960
Flomenberg PR / 1987 / Molecular epidemiology of adenovirus type 35 infections in immunocompromised hosts / J Infect Dis 155:1127~1134
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UCI(KEPA) : I410-ECN-0101-2013-510-000796387