지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.9
- 수록면
- 191 - 196 (6page)
이용수
초록· 키워드
오류제보하기
Noroviruses (noroV) are the major cause of nonbacterial gastroenteritis in humans worldwide. Since noroV cannot yet be cultured in vitro and their diagnosis by electron microscopy requires at least 10<sup>6</sup> viral particles/g of stool a variety of molecular detection techniques represent an important step towards the detection of noroV. In the present study, we have applied real-time nucleic acid sequence-based amplification (real-time NASBA) for simultaneous detection of NoroV genogroup Ⅰ (GⅠ) and genogroup Ⅱ (GⅡ) using standard viral RNA. For real-time NASBA assay which can detected noroV GⅠ and GⅡ, a selective region of the genes encoding the capsid protein was used to design primers and genotype-specific molecular beacon probes. The specificity of the real-time NASBA using newly designed primers and probes were confirmed using standard viral RNA of noroV GⅠ and GⅡ. To determine the sensitivity of this assay, serial 10-fold dilutions of standard viral RNA of noroV GⅠ and GⅡ were used for reverse transcription polymerase chain reaction (RT-PCR) and real-time NASBA. The results showed that while agarose gel electrophoresis could detect RT-PCR products with 10 pg of standard viral RNA, the real-time NASBA assay could detect 100 fg of standard viral RNA. These results suggested that the real-time NASBA assay has much higher sensitivity than conventional RT-PCR assay. This assay was expected that might detect the viral RNA in the specimens which could have been false negative by RT-PCR. There were needed to perform real-time NASBA with clinical specimens for evaluating accurate sensitivity and specificity of this assay.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
참고문헌 (15)
참고문헌 신청
Chan MC / 2006 / Fecal viral load and nororivus-associated gastroenteritis / Emerg Infect Dis 12:1278~1280
Fankhauser RL / 1998 / Molecular epidemiology of "Norwalk-like viruses" in outbreaks of gastroenteritis in the United States / J. Infect. Dis 178:1571~1578
Fankhauser RL / 2002 / Epidemiologic and molecular trends of "norwalklike viruses" associated with outbreaks of gastroenteritis in the United States / J Infect Dis 186:1~7
Koopmans M / 2002 / Foodborne viruses / FEMS Microbiol Rev 26:187~205
Koopmans M / 2008 / Progress in understanding norovirus epidemiology / Curr Opin Infect Dis 21:544~552
Fankhauser RL / 1998 / Molecular epidemiology of "Norwalk-like viruses" in outbreaks of gastroenteritis in the United States / J. Infect. Dis 178:1571~1578
Fankhauser RL / 2002 / Epidemiologic and molecular trends of "norwalklike viruses" associated with outbreaks of gastroenteritis in the United States / J Infect Dis 186:1~7
Koopmans M / 2002 / Foodborne viruses / FEMS Microbiol Rev 26:187~205
Koopmans M / 2008 / Progress in understanding norovirus epidemiology / Curr Opin Infect Dis 21:544~552
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UCI(KEPA) : I410-ECN-0101-2013-510-000796392