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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.6
- 수록면
- 508 - 513 (6page)
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초록· 키워드
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Erythromycin has been used to treat Streptococosis, Edwardsiellosis, Vibriosis, Bacterial enteritis in the cultured fish. In this study, a rapid and effective erythromycin analysis method with new sample treatment protocol and liquid chromatography/tandem mass spectrometry (LC/MS/MS) system for fish products was developed. For the erythromycin extraction from fish muscle, the solvent mixture composed of 0.2% meta-phosphoric acid and methanol (6:4) showed good recovery rate, and the optimum extraction solvent volume was 20 mL. Erythromycin detection using LC/MS/MS were carried out under electrospray ionization (ESI) positive condition and erythromycin mass value 576.2 and 157.9. And the detection limit of the established method was 0.005 mg/kg in fish products. The recovery rate of the developed method applied to the fish species were as following, olive flounder, 87.6±5.0%; black rockfish, 87.2±6.4%; eel, 85.2±4.8%; and rainbow trout, 86.0±6.2%. In the established method in this study, the correlation of coefficient values (R2) of erythromycin calibration curve (n=11) was 0.9998.
목차
Abstract
Introduction
Material and Methods
Results and Discussion
References
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UCI(KEPA) : I410-ECN-0101-2013-573-001009153