This paper is concerned with the ATO speed profile generation method in the moving block system. The ATO speed profile is calculated using the acceleration limit and the jerk limit to improve ride comfort. In addition, the speed limit and stop distance provided from ATP profile should be considered to ensure safety. In the moving block system, the speed limit and the stop position are frequently changed in real-time. Therefore, the ATO speed profile should be regenerated immediately according to change of the speed limit and the stop position, within the acceleration limit and the jerk limit. In this paper, the ATO speed profile generation method is proposed, which considers not only frequent changes of the speed limit and the stop position but also acceleration limit and jerk limit. Futhermore the simulation result is presented to verify usefulness of this method.