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논문 기본 정보

자료유형
학술저널
저자정보
Yelim Jang (서울대학교) Sang Gyu Kim (서울대학교) Young Ho Kim (서울대학교)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal Vol.27 No.4
발행연도
2011.12
수록면
333 - 341 (9page)

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초록· 키워드

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Two antifungal bacteria were selected from forest soils during the screening of microorganisms antagonistic to Cylindrocarpon destructans, a cause of ginseng root rot. The antifungal bacteria were identified as Bacillus subtilis (I4) and B. amyloliquefaciens (yD16) based on physiological and cultural characteristics, the Biolog program, and 16S rRNA gene sequencing analyses. Antagonistic activity of both bacterial isolates to C. destructans increased with increasing temperature. More rapid starch hydrolytic activity of the bacteria was seen on starch agar at higher temperatures than at lower temperatures, and in the higher density inoculum treatment than in the lower density inoculum treatment. The bacterial isolates failed to colonize ginseng root the root tissues inoculated with the bacteria alone at an inoculum density of 1 × 10? cfu/㎖, but succeeded in colonizing the root tissues co-inoculated with the bacteria and C. destructans. Scanning electron microscopy showed that the pathogen was damaged by the low-density inoculum treatment with the bacterial isolates as much as by the high-density inoculum treatment. Both bacterial isolates were more effective in reducing root rot when they were treated at a concentration of 1 × 10? cfu/㎖ than at 1 × 10? cfu/㎖. Also, only the former treatment induced prominent wound periderm formation, related to structural defense against pathogen infection. The results suggest that the bacterial antagonists may have high potential as biocontrol agents against ginseng root rot at relatively low-inoculum concentrations.

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Materials and Methods
Results
Discussion
Acknowledgement
References

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UCI(KEPA) : I410-ECN-0101-2013-481-001446075