지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.12
- 수록면
- 339 - 342 (4page)
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초록· 키워드
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Mutations in the transmembrane inner ear (Tmie) gene, which encodes the Tmie protein, have been attributed to deafness autosomal recessive 6 (DFNB6), an autosomal nonsyndromic recessive hearing loss disorder. Although the Tmie gene was identified a few years ago, little is known about subcellular localization of the Tmie protein. In order to address this, we developed a stable cell line expressing Tmie protein. The expression of Myc-tagged Tmie protein was confirmed by Western blot analysis using an anti-Myc antibody and localization of the Tmie protein was confirmed by immunostaining, using the anti-Myc antibody as well as the anti-tmie antibody. Our study demonstrates that the Tmie protein is localized mostly in the cellular membrane and to a lesser extent in cytoplasm. These results suggest that our Tmie expressing stable cell line provides a suitable in vitro model to explore Tmie synthesis and functions.
목차
Materials and Methods
Results
Discussion
Acknowledgments
References
참고문헌 (16)
참고문헌 신청
Dror AA / 2010 / Hearing impairment: a panoply of genes and functions / Neuron 68(2):293~308
Raviv D / 2010 / Hearing loss: a common disorder caused by many rare alleles / Ann N Y Acad Sci 1214:168~179
Lalwani AK / 1999 / Cracking the Auditory Genetic Code: nonsyndromic hereditary hearing impairment / Am J Otol 20:115~132
Naz S / 2002 / Mutations in a novel gene, TMIE, are associated with hearing loss linked to the DFNB6 locus / Am J Hum Genet 71(3):632~636
Mitchem KL / 2002 / Mutation of the novel gene Tmie results in sensory cell defects in the inner ear of spinner, a mouse model of human hearing loss DFNB6 / Hum Mol Genet 11(6):1887~1898
Raviv D / 2010 / Hearing loss: a common disorder caused by many rare alleles / Ann N Y Acad Sci 1214:168~179
Lalwani AK / 1999 / Cracking the Auditory Genetic Code: nonsyndromic hereditary hearing impairment / Am J Otol 20:115~132
Naz S / 2002 / Mutations in a novel gene, TMIE, are associated with hearing loss linked to the DFNB6 locus / Am J Hum Genet 71(3):632~636
Mitchem KL / 2002 / Mutation of the novel gene Tmie results in sensory cell defects in the inner ear of spinner, a mouse model of human hearing loss DFNB6 / Hum Mol Genet 11(6):1887~1898
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UCI(KEPA) : I410-ECN-0101-2013-510-001228171