Several studies indicated that a rest frame reduced cybersickness, especially the vection induced sickness, associated with exposure to real 3D virtual environments. The form and the way realizing the rest frame in 3D virtual environments have different effects on immersion, presence and reducing cybersickness. Therefore it is important to find the most effective form and method realizing a rest frame in 3D virtual environments reducing cybersickness while at the same time maintaining presence and immersion. In this study, we realized a 3D rectangulartunnel rest frame and a real 3D virtual environment inducing cybersickness. For verifying the rest frame’s effect of reducing cybersickness, 10 subjects were exposed to both rest and nonrest conditions after an interval of three days after the real 3D virtual environment. We recorded electrogastrogram, skin conductance, heart rate, and respiration during the exposure to 3D virtual environment. Simulator sickness questionnaire(SSQ) was used for measuring the intensity of the sickness before and after the exposure. The results showed that the scores of SSQ were not reduced significantly by using the rest frame. All 4 electrophysiological responses were not changed significantly from the baseline period to the exposure of 3D virtual environment in the rest frame condition compared to the nonrest frame condition. The results suggest that 3D rectangulartunnel rest frame is not an adapted rest frame for reducing cybersickness in real 3D virtual environments.