Effects of Long-Term High-Fat Diet Feeding on Gene Expression of Inflammatory Cytokines in Mouse Adipose Tissue

Nu Ri Oh; Ae Rang Hwang; Ja In Jeong; Sung Hyun Park; Jin Seok Yang; Yong-Ho Lee

추천

검색

질문

자료유형: 학술저널

저자정보: Nu Ri Oh (Catholic University of Daegu) Ae Rang Hwang (Catholic University of Daegu) Ja In Jeong (Catholic University of Daegu) Sung Hyun Park (Catholic University of Daegu) Jin Seok Yang (Catholic University of Daegu) Yong-Ho Lee (Catholic University of Daegu)

저널정보: 대한의생명과학회 대한의생명과학회지 대한의생명과학회지 제18권 제1호

발행연도: 2012.3

수록면: 56 - 62 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

This study was to investigate the effects of high-fat diet feeding for a very long period of time on gene expression of inflammatory cytokines in mouse adipose tissue and to determine whether caloric restriction (CR) or insulin sensitizer treatment changes the cytokine gene expressions even in obese mice fed a high-fat diet for a very long term-period. Gene expression levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 (MCP-1) were examined by real-time PCR in subcutaneous abdominal adipose tissue (SubQ) from obese and non-obese male C57BL/6 mice at 16, 26, 36, 47, and 77 weeks of age on either normal diet (ND) or high-fat diet (HFD) after starting at 6 weeks of age. In addition, gene expression levels of TNF-α, IL-6 and MCP-1 were determined in SubQ before and after rosiglitazone treatment or CR on 47-week-old obese mice. The results demonstrated that gene expression levels of TNF-α, IL-6 and MCP-1 were significantly increased with aging in SubQ of mice in both groups of diet. MCP-1 gene expression of SubQ in all ages tested was significantly or marginally increased in mice on HFD compared with ND. While TNF-α expression was significantly reduced by rosiglitazone, IL-6 and MCP-1 were significantly decreased by CR. The basic data in this study will be useful for characterizing the C57BL/6 mouse as an animal model of obesity induced by high-fat diet feeding for a very long period of time, and a better understanding of inflammatory cytokine regulation in diet induced obesity which may facilitate the development of new therapeutic strategies to prevent the complications of obesity.

#Obesity #High-fat diet #TNF-α #IL-6 #MCP-1 #Adipose tissue #C57BL/6 #Caloric restriction #Rosiglitazone

참고문헌 (21)

참고문헌 신청

Alkhouri N / 2010 / Adipocyte apoptosis, a link between obesity, insulin resistance, and hepatic steatosis / J Biol Chem 285:3428~3438

Altintas MM / 2011 / Apoptosis, mastocytosis, and diminished adipocytokine gene expression accompany reduced epididymal fat mass in long-standing diet-induced obese mice / Lipids in Health and Disease 10:198

Higuchi A / 2010 / Thiazolidinediones reduce pathological neovascularization in ischemic retina via an adiponectin-dependent mechanism / Arterioscler Thromb Vasc Biol 30:46~53

Hotamisligil GS / 1993 / Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance / Science 259:87~91

Hotamisligil GS / 1994 / Tumor necrosis factor alpha: a key component of the obesity-diabetes link / Diabetes 43:1271~1278