지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.8
- 수록면
- 85 - 95 (11page)
이용수
초록· 키워드
오류제보하기
Purpose: Diallyl disulfide (DADS) is a major organosulfur compound derived from garlic. It has been reported that DADS is able to inhibit the proliferation of several tumor cells. In this study, the effect of DADS was investigated in terms of the proliferation of AGS, gastric adenocarcinoma cell line at various concentrations. Methods: The viability of cultured cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. To detect the induction of apoptosis, Annexin V-FITC/propodium iodide (PI) staining assay was performed. Analysis of reactive oxygen species (ROS) and the distribution of cells in the cell cycle were measured by a flow cytometer. And using the Western blot analysis, the change of Fas, caspase-3, Bax, Bcl-2 activity was measured. Results: The percentage of live AGS cells was decreased to 23% of that in the control group after 400 μM DADS treatment for 48 hours. The Annexin V positive/PI negative (apoptosis portion) area increased from low concentration of DADS to high concentration. When comparing among the DADS treatment groups, the amount of ROS production increased in a dose dependent manner. The percentage of sub-diploid DNA content increased from 8.71% at 50 μM to 25.74% at 400 μM DADS treatment group. The expressions of Fas, caspase-3, Bax were increased and that of Bcl-2 was decreased in a dose dependent manner. Conclusion: DADS decreases the viability of AGS cell lines and induces apoptosis in a dose-dependent manner. But the relationship of the anti-proliferative effect of DADS and related molecular changes were not clearly proportional to the concentration of DADS.
목차
Purpose
INTRODUCTION
METHODS
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
REFERENCES
참고문헌 (29)
참고문헌 신청
1. [학술지(정기간행물)] - Sun SY / 2004 / Apoptosis as a novel target for cancer chemoprevention / J Natl Cancer Inst 96 : 662 ~ 672
2. [학술지(정기간행물)] - Fleischauer AT / 2001 / Garlic and cancer: a critical review of the epidemiologic literature / J Nutr 131 (3S) : 1032S ~ 1040S
3. [학술지(정기간행물)] - Wargovich MJ / 1996 / Allium vegetables: their role in the prevention of cancer / Biochem Soc Trans 24 : 811 ~ 814
4. [학술지(정기간행물)] - Milner JA / 2001 / A historical perspective on garlic and cancer / J Nutr 131 (3S) : 1027S ~ 1031S
5. [학술지(정기간행물)] - Khanum F / 2004 / Anticarcinogenic properties of garlic: a review / Crit Rev Food Sci Nutr 44 : 479 ~ 488
2. [학술지(정기간행물)] - Fleischauer AT / 2001 / Garlic and cancer: a critical review of the epidemiologic literature / J Nutr 131 (3S) : 1032S ~ 1040S
3. [학술지(정기간행물)] - Wargovich MJ / 1996 / Allium vegetables: their role in the prevention of cancer / Biochem Soc Trans 24 : 811 ~ 814
4. [학술지(정기간행물)] - Milner JA / 2001 / A historical perspective on garlic and cancer / J Nutr 131 (3S) : 1027S ~ 1031S
5. [학술지(정기간행물)] - Khanum F / 2004 / Anticarcinogenic properties of garlic: a review / Crit Rev Food Sci Nutr 44 : 479 ~ 488
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2013-514-002683921