지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 404 - 411 (8page)
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Purpose: Recently, two alternatively spliced survivin variants, survivin-ΔEx3 and survivin-2B, were identified in a single copy of the survivin gene. It has been reported that the expressions of survivin splice variants significantly correlates with the clinical results in many types of human carcinoma. We investigated the transcription levels of survivin and its splice variants in human colorectal carcinomas, and analyzed correlations between survivin expression levels and clinicopathologic features. Methods: We used Western blot and real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) to analyze the protein and mRNA expression levels of survivin variants in 51 colorectal carcinomas. The quantitative RT-PCR was performed using primer pairs specific for survivin and each of its splice variants, then normalized for the gene that encodes glyceraldehydes-3-phosphate dehydrogenase. Results: In Western blotting, the protein levels of survivin were higher in the tumor tissue than in normal tissue. The expression of survivin, survivin-2B and survivin-ΔEx3 mRNA was present in 96%, 64.7%, and 82.4% of the samples, respectively. When the pathologic parameters were compared, colorectal cancers of advanced pT stages showed significant decrease in survivin-2B mRNA expression by the quantitative RT-PCR (P ＜ 0.001). Conclusion: The decreased expression of survivin-2B might be related to tumor progression in colorectal cancers. This finding indicates that alternatively spliced variants of survivin may be involved in refining the functions of survivin during tumor progression.
목차
Purpose
INTRODUCTION
METHODS
RESULTS
DISCUSSION
CONFLICTS OF INTEREST
REFERENCES
참고문헌 (26)
참고문헌 신청
1. [학술지(정기간행물)] - Modrek B / 2002 / A genomic view of alternative splicing / Nat Genet 30 : 13 ~ 19
2. [학술지(정기간행물)] - Ng CC / 1999 / Isolation and characterization of a novel TP53-inducible gene, TP53TG3 / Genes Chromosomes Cancer 26 : 329 ~ 335
3. [학술지(정기간행물)] - Weng MW / 2005 / qAlternative splicing of MDM2 mRNA in lung carcinomas and lung cell lines / Environ Mol Mutagen 46 : 1 ~ 11
4. [학술지(정기간행물)] - Yukitake H / 2002 / AAT-1, a novel testis-specific AMY-1-binding protein, forms a quaternary complex with AMY-1, A-kinase anchor protein 84, and a regulatory subunit of cAMP-dependent protein kinase and is phosphorylated by its kinase / J Biol Chem 277 : 45480 ~ 45492
5. [학술지(정기간행물)] - Ambrosini G / 1997 / A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma / Nat Med 3 : 917 ~ 921
2. [학술지(정기간행물)] - Ng CC / 1999 / Isolation and characterization of a novel TP53-inducible gene, TP53TG3 / Genes Chromosomes Cancer 26 : 329 ~ 335
3. [학술지(정기간행물)] - Weng MW / 2005 / qAlternative splicing of MDM2 mRNA in lung carcinomas and lung cell lines / Environ Mol Mutagen 46 : 1 ~ 11
4. [학술지(정기간행물)] - Yukitake H / 2002 / AAT-1, a novel testis-specific AMY-1-binding protein, forms a quaternary complex with AMY-1, A-kinase anchor protein 84, and a regulatory subunit of cAMP-dependent protein kinase and is phosphorylated by its kinase / J Biol Chem 277 : 45480 ~ 45492
5. [학술지(정기간행물)] - Ambrosini G / 1997 / A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma / Nat Med 3 : 917 ~ 921
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UCI(KEPA) : I410-ECN-0101-2013-514-002684064