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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 255 - 261 (7page)
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초록· 키워드
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A bacterium AB-55, isolated from waste mushroom bed of Agaricus bisporus in Sukseong-myeon, Buyeo-gun, Chungcheongnam-do, Korea, was screened onto xylan agar congo-red plate by the xylanolysis method and was used to produce an xylanase in shaker buffle flask cultures containing oat spelt xylans. The phylogenetic analysis using 16S rRNA gene sequence data showed that the strain AB-55 had the highest homology (99.0%) with Bacillus subtilis and it was named as Bacillus subtilis AB-55. A xylanase was purified by ammonium sulfate precipitation (50∼80%), gel filtration on sephacryl S-300, and ion exchange chromatography on DEAE sepharose FF. The molecular weight of the xylanase was estimated as 44 kDa by SDS-PAGE. Optimal pH and temperature for the xylanase activity was pH 7 and 50℃, respectively. N-terminal amino acid sequence of the enzyme was identified as Ser-Ala-Val-Lys-His-Gly-Ala-Ile-Val-Phe. The substrate specificity of the enzyme exhibited that it hydrolyzed efficiently oat spelt xylan as well as beechwood xylan, but showed no activity against Avicel and carboxymethyl clellulose (CMC). The enzyme activity was enhanced by Fe<SUP>2+</SUP> and Mn<SUP>2+</SUP>whereas was entirely inhibited by Hg?.
목차
Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과 및 고찰
Ⅳ. 결론
감사의 글
참고문헌
참고문헌 (27)
참고문헌 신청
Anthony L / 2005 / Overproduction and characterization of xylanase B from Aspergillus niger / Can. J. Microbiol 51:177~183
Bailey MJ / 1992 / testing of methods for assay of xylanase activity / J. Biotechnol 23:257~270
Beldman G / 1985 / The cellulase of Trichoderma viride: Purification, characterization and comparison of all detectable endoglucanase, exoglucanase and β-glucosidase / Eur. J. Biochem 146:301~308
Chidi SB / 2008 / Production, purification and characterisation of cellulase free xylanase from Aspergillus terrus UL 4209 / Afr. J. Biotecnol 7:3939~3948
Chun JJ / 2007 / Ex Taxonia web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence / Int. J. Syst. Evol. Microbiol 57:2259~2261
Bailey MJ / 1992 / testing of methods for assay of xylanase activity / J. Biotechnol 23:257~270
Beldman G / 1985 / The cellulase of Trichoderma viride: Purification, characterization and comparison of all detectable endoglucanase, exoglucanase and β-glucosidase / Eur. J. Biochem 146:301~308
Chidi SB / 2008 / Production, purification and characterisation of cellulase free xylanase from Aspergillus terrus UL 4209 / Afr. J. Biotecnol 7:3939~3948
Chun JJ / 2007 / Ex Taxonia web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence / Int. J. Syst. Evol. Microbiol 57:2259~2261
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UCI(KEPA) : I410-ECN-0101-2013-480-002670341