지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 104 - 111 (8page)
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초록· 키워드
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Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases which degrade extracellular matrix (ECM) during embryogenesis, wound healing, and tissue remodeling. Dysregulation of MMP activity is also associated with various pathological inflammatory conditions. In this study, we examined the expression pattern of MMPs during PMA-induced differentiation of THP-1 monocytic cells into macrophages. We found that MMP1, MMP8, MMP3, MMP10, MMP12, MMP19, MMP9, and MMP7 were upregulated during differentiation whereas MMP2 remained unchanged. Expression of MMPs increased in a time-dependent manner; MMP1, MMP8, MMP3, MMP10, and MMP12 increased beginning at 60 hr post PMA treatment whereas MMP19, MMP9, and MMP7 increased beginning at 24 hr post PMA treatment. To identify signal transduction pathways involved in PMA-induced upregulation of MMPs, we treated PMA-differentiated THP-1 cells with specific inhibitors for PKC, MEK1, NF-κB, PI3K, p38 MAPK and PLC. We found that inhibition of the MEK1 pathway blocked PMA-induced upregulation of all MMPs to varying degrees except for MMP-2. In addition, expression of select MMPs was inhibited by PI3K, p38 MAPK and PLC inhibitors. In conclusion, we show that of the MMPs examined, most MMPs were up-regulated during differentiation of monocyte into macrophage via the MEK1 pathway. These results provide basic information for studying MMPs expression during macrophage differentiation.
목차
서론
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (30)
참고문헌 신청
Amorino GP / 1998 / Interactions of monocytic cells with human endothelial cells stimulate monocytic metalloproteinase production / Am J Pathol 152:199~207
Andrea S / 2010 / Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines / BMC Cancer 10:553~564
Andrew CN / 2008 / Metalloproteinases expression in monocytes and macrophages and its relationship to atherosclerotic plaque instability / Arterioscler Thromb Vasc Biol 28:2108~2114
Ardans JA / 2002 / Oxidized low-density and high-density lipoproteins regulate the production of matrix metalloproteinase-1 and -9 by activated monocytes / J Leukoc Biol 71:1012~1018
Bar-Or A / 2003 / Analyses of all matrix metalloproteinase members in leukocytes emphasize monocytes as major inflammatory mediators in multiple sclerosis / Brain 126:2738~2749
Andrea S / 2010 / Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines / BMC Cancer 10:553~564
Andrew CN / 2008 / Metalloproteinases expression in monocytes and macrophages and its relationship to atherosclerotic plaque instability / Arterioscler Thromb Vasc Biol 28:2108~2114
Ardans JA / 2002 / Oxidized low-density and high-density lipoproteins regulate the production of matrix metalloproteinase-1 and -9 by activated monocytes / J Leukoc Biol 71:1012~1018
Bar-Or A / 2003 / Analyses of all matrix metalloproteinase members in leukocytes emphasize monocytes as major inflammatory mediators in multiple sclerosis / Brain 126:2738~2749
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UCI(KEPA) : I410-ECN-0101-2013-510-002648595