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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.6
- 수록면
- 165 - 168 (4page)
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Hox proteins containing DNA-binding homedomain act as transcription factors important for anteroposterior body patterning during vertebrate embryogenesis. However, the precise mechanisms by which signal pathways are transduced to regulate the Hox gene expression are not clear. In the course of an attempt to isolate an upstream regulatory factor(s) controlling Hox genes, protein kinase B alpha (Akt1) has been identified as a putative regulator of Hox genes through in silico analysis (GEO profile). In the Gene Expression Omnibus (GEO) dataset GDS1784 at the NCBI (National Center for Biotechnology Information) site, Hox genes were differentially expressed depending on the presence or absence of Akt1. Since it was not well known how Akt1 regulates the specific Hox genes, whose transcription was reported to be regulated by epigenetic modifications such as histone acetylation, methylation etc., the expression of Gcn5, a histone acetyltransferase (HAT), was analyzed in wild type (WT) as well as in Akt1-/- mouse embryonic fibroblast (MEF) cells. RT-PCR analysis revealed that the amount of Gcn5 mRNA was similar in both WT and Akt1-/- MEFs. However, the protein level of Gcn5 was significantly increased in Akt1-/- MEF cells. The half life of Gcn5 was 1 hour in wild type whereas 8 hours in Akt1-/- MEF. These data all together, indicate that Gcn5 is post-transcriptionally down-regulated and the protein stability is negatively regulated by Akt1 in MEF cells.
목차
Acknowledgements
REFERENCES
참고문헌 (14)
참고문헌 신청
Barber BA / 2010 / Epigenetic control of Hox genes during neurogenesis, development, and disease / Ann Anat 192:261~274
Brownell JE / 1996 / Tetrahymena histone acetyltransferase A: A homolog to yeast Gcn5p linking histone acetylation to gene activation / Cell 84:843~851
Cho H / 2001 / Akt1/ PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice / J Biol Chem 276:38349~38352
Deschamps J / 2005 / Developmental regulation of the Hox genes during axial morphogenesis in the mouse / Development 132:2931~2942
Favier B / 1997 / Developmental functions of mammalian Hox genes / Mol Hum Reprod 3:115~131
Brownell JE / 1996 / Tetrahymena histone acetyltransferase A: A homolog to yeast Gcn5p linking histone acetylation to gene activation / Cell 84:843~851
Cho H / 2001 / Akt1/ PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice / J Biol Chem 276:38349~38352
Deschamps J / 2005 / Developmental regulation of the Hox genes during axial morphogenesis in the mouse / Development 132:2931~2942
Favier B / 1997 / Developmental functions of mammalian Hox genes / Mol Hum Reprod 3:115~131
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