지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.6
- 수록면
- 107 - 116 (10page)
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Purpose: This study is designed to ascertain the most effective quantity and injection route of hepatocytes in an acute liver failure model induced by massive liver resection in rats.
Methods: Rats weighing 450 to 650 gm underwent partial hepatectomy that was 80% of their liver weight, resulting in acute liver failure. Hepatocytes were obtained by perfusing collagenase (Wako, Japan) solution through portal vein into liver of the allogenic rat. These hepatocytes were injected into different places with different dosage. The experimental groups were divided into the Control group, Splenic group I (2×106 cells into splenic capsule), Splenic group II (2×107 cells into splenic capsule), Portal vein group (2×107 cells into portal vein), Subperitoneal group (2×107 cells into subperitoneum). The experimental animals were observed carefully for 5 days for assessment of survival and regeneration of liver. Liver function tests including serum alanine aminotransferase (ALT), total bilirubin, gamma-glutamyl transferase (γ-GTP) on postoperative 1, 2, 3, 5th days and histologic examinations of specimens obtained from each respective groups on postoperative 5th day were performed.
Results: Serum ALT level on postoperative day 1 peaked and then gradually normalized showing statistical significance (p=0.035). Study groups showing statistically significant difference under repeated anova analysis were between the Splenic group II and Control (p=0.035), and between the Splenic group II and Portal vein group (p=0.001) with respect to serum ALT levels. Also, progression of each study group showed statistical significance. (p=0.02). Serum total bilirubin and r-GTP did not show any significant difference.
Conclusion: Hepatocyte transplantation of 2×107 cells into spleen showed the best results in the acute hepatic failure rat.
Methods: Rats weighing 450 to 650 gm underwent partial hepatectomy that was 80% of their liver weight, resulting in acute liver failure. Hepatocytes were obtained by perfusing collagenase (Wako, Japan) solution through portal vein into liver of the allogenic rat. These hepatocytes were injected into different places with different dosage. The experimental groups were divided into the Control group, Splenic group I (2×106 cells into splenic capsule), Splenic group II (2×107 cells into splenic capsule), Portal vein group (2×107 cells into portal vein), Subperitoneal group (2×107 cells into subperitoneum). The experimental animals were observed carefully for 5 days for assessment of survival and regeneration of liver. Liver function tests including serum alanine aminotransferase (ALT), total bilirubin, gamma-glutamyl transferase (γ-GTP) on postoperative 1, 2, 3, 5th days and histologic examinations of specimens obtained from each respective groups on postoperative 5th day were performed.
Results: Serum ALT level on postoperative day 1 peaked and then gradually normalized showing statistical significance (p=0.035). Study groups showing statistically significant difference under repeated anova analysis were between the Splenic group II and Control (p=0.035), and between the Splenic group II and Portal vein group (p=0.001) with respect to serum ALT levels. Also, progression of each study group showed statistical significance. (p=0.02). Serum total bilirubin and r-GTP did not show any significant difference.
Conclusion: Hepatocyte transplantation of 2×107 cells into spleen showed the best results in the acute hepatic failure rat.
목차
Introduction
Methods
Results
Discussion
Conclusion
References
참고문헌 (23)
참고문헌 신청
1. [학술지(정기간행물)] - Gill RQ / 2001 / Acute liver failure / J Clin Gastroenterol 33 : 191 ~ 198
2. [학술지(정기간행물)] - Gotthardt D / 2007 / Fulminant hepatic failure: etiology and indications for liver transplantation / Nephrol Dial Transplant 22 (Suppl8) : viii5 ~ viii8.
3. [학술지(정기간행물)] - Debray D / 2006 / New management options for end-stage chronic liver disease and acute liver failure: potential for pediatric patients / Paediatr Drugs 8 : 1 ~ 13
4. [학술지(정기간행물)] - Kjaergard LL / 2003 / Artificial and bioartificial support systems for acute and acute-on-chronic liver failure: a systematic review / JAMA 289 : 217 ~ 222
5. [학술지(정기간행물)] - Demetriou AA / 2004 / Prospective, randomized, multicenter, controlled trial of a bioartificial liver in treating acute liver failure / Ann Surg 239 : 660 ~ 667
2. [학술지(정기간행물)] - Gotthardt D / 2007 / Fulminant hepatic failure: etiology and indications for liver transplantation / Nephrol Dial Transplant 22 (Suppl8) : viii5 ~ viii8.
3. [학술지(정기간행물)] - Debray D / 2006 / New management options for end-stage chronic liver disease and acute liver failure: potential for pediatric patients / Paediatr Drugs 8 : 1 ~ 13
4. [학술지(정기간행물)] - Kjaergard LL / 2003 / Artificial and bioartificial support systems for acute and acute-on-chronic liver failure: a systematic review / JAMA 289 : 217 ~ 222
5. [학술지(정기간행물)] - Demetriou AA / 2004 / Prospective, randomized, multicenter, controlled trial of a bioartificial liver in treating acute liver failure / Ann Surg 239 : 660 ~ 667
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UCI(KEPA) : I410-ECN-0101-2014-514-001274493