Tip burn has been reported as one of the most serious physiological disorder in Chinese chives (Allium tuberosum Rottl.) cultivated in plastic film house. In this study, a physiography and chemical properties of 132 plastic film house soils were investigated to elucidate factors affecting tip burn symptom. Also influence of soil properties on tip burn was statistically determined by path analysis and association analysis including a chi-square test or logistics analysis. Probability distribution of inorganic aqueous species, such as ammonia (g) was calculated using MINTEQ program. Soil order and chemical properties, especially pH, exchangeable calcium and inorganic nitrogen, showed a significant relationship with tip burn of Chinese chives. Tip burn symptoms occur mainly in an alkaline soil classified as Alfisols. Result of linear regression and path analysis exhibited that formation of ammonia (g) from soil solution depend upon soil pH and were associated with ammonium resulting from soil organic matter or nitrate. These results indicate that tip burn symptom of Chinese chives is directly affected by ammonia gas originated from alkaline soil condition.