지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 104 - 111 (8page)
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초록· 키워드
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Objectives: Marijuana is one of the most frequently abused drug in Korea and its adverse health effects are controversial. p53 is known to be crucial in regulating the DNA damage responses, and adverse effects can occur when it is regulated by marijuana smoke. We evaluated a role of p53 on genotoxic effect and apoptosis in lung cancer cells exposed to marijuana smoke condensates (MSCs).
Methods: The p53-related genotoxicity and apoptosis of MSCs were evaluated using in vitro bioassay, viz., comet assay, cytokinesis-block micronucleus assay and apoptosis assay. We used two cell lines with differential p53 expression (p53-wildtype (WT) H460 and p53-null H1299).
Results: MSCs significantly increased DNA breakages and chromosomal changes in p53-WT H460 and p53-null H1299 cells. The genotoxicity induced by MSCs in p53-null H1299 cells showed greater sensitivity than p53-WT H460 cells. Moreover, MSCs showed a significant increase in reactive oxygen species production and apoptosis. The apoptotic responses induced by MSCs were higher in p53-WT H460 cells than in p53-null H1299 cells. Significantly increased mRNA expression or apoptosis related genes, including p53, caspase-3, and Bax/Bcl-2 ratio were observed in the p53-WT H460 cells exposed to MSCs.
Conclusions: These results suggest that MSCs induce DNA/chromosomal damages and apoptosis in human lung cancer cells and p53 plays an important role in the cellular response to MSCs. The present study may have border implications for our understanding of pulmonary diseases.
Methods: The p53-related genotoxicity and apoptosis of MSCs were evaluated using in vitro bioassay, viz., comet assay, cytokinesis-block micronucleus assay and apoptosis assay. We used two cell lines with differential p53 expression (p53-wildtype (WT) H460 and p53-null H1299).
Results: MSCs significantly increased DNA breakages and chromosomal changes in p53-WT H460 and p53-null H1299 cells. The genotoxicity induced by MSCs in p53-null H1299 cells showed greater sensitivity than p53-WT H460 cells. Moreover, MSCs showed a significant increase in reactive oxygen species production and apoptosis. The apoptotic responses induced by MSCs were higher in p53-WT H460 cells than in p53-null H1299 cells. Significantly increased mRNA expression or apoptosis related genes, including p53, caspase-3, and Bax/Bcl-2 ratio were observed in the p53-WT H460 cells exposed to MSCs.
Conclusions: These results suggest that MSCs induce DNA/chromosomal damages and apoptosis in human lung cancer cells and p53 plays an important role in the cellular response to MSCs. The present study may have border implications for our understanding of pulmonary diseases.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
CONFLICT OF INTEREST
REFERENCES
참고문헌 (40)
참고문헌 신청
Chung H / 2004 / Recent trends of drug abuse and drug-associated deaths in Korea / Ann N Y Acad Sci 1025:458~464
Soo Yeun Lee / 2005 / Antiestrogenic Effects of Marijuana Smoke Condensate and Cannabinoid Compounds / Archives of Pharmacal Research 28(12):1365~1375
Hoffmann D / 1975 / On the carcinogenicity of marijuana smoke / Recent Adv Phytochem 9:63~81
Novotny M / 1982 / Fractionation and capillary gas chromatographic mass spectrometric characterization of the neutral components in marijuana and tobacco smoke concentrates / J Chromatogr 238(1):141~150
Tashkin DP. / 2005 / Smoked marijuana as a cause of lung injury / Monaldi Arch Chest Dis 63(2):93~100
Soo Yeun Lee / 2005 / Antiestrogenic Effects of Marijuana Smoke Condensate and Cannabinoid Compounds / Archives of Pharmacal Research 28(12):1365~1375
Hoffmann D / 1975 / On the carcinogenicity of marijuana smoke / Recent Adv Phytochem 9:63~81
Novotny M / 1982 / Fractionation and capillary gas chromatographic mass spectrometric characterization of the neutral components in marijuana and tobacco smoke concentrates / J Chromatogr 238(1):141~150
Tashkin DP. / 2005 / Smoked marijuana as a cause of lung injury / Monaldi Arch Chest Dis 63(2):93~100
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UCI(KEPA) : I410-ECN-0101-2014-539-000804734