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논문 기본 정보

자료유형
학술저널
저자정보
Ni Van (University of Illinois at Chicago) Ned Ahlberg (University of Illinois at Chicago) Byung Chul Jung (Yonsei University) Min Ho Lee (Yonsei University) Seung Ju Ahn (Daegu Health College) In-soo Lee (Hyejeon College) Yoon Suk Kim (Yonsei University) Ki-Jong Rhee (Yonsei University)
저널정보
대한의생명과학회 대한의생명과학회지 대한의생명과학회지 제18권 제4호
발행연도
2012.12
수록면
362 - 368 (7page)

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초록· 키워드

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Enterotoxigenic Bacteroides fragilis (ETBF) is an intestinal commensal bacterium implicated as a risk factor for colon cancer. The key virulence factor is a secreted toxin called B. fragilis toxin (BFT). In this study we used an in vitro bioassay to examine the prevalence of ETBF in colonic washings from patients with colorectal polyps and normal control patients. We found that 9.3% of polyp patients and 10.9% of non-polyp patients harbored ETBF, respectively. A total of nine ETBF clinical isolates were isolated and confirmed to be positive for the BFT gene by PCR analysis and the ability to induce IL-8 secretion in the colonic epithelial cell line HT29/c1. Two of the ETBF clinical strains were characterized further in vitro and in vivo. We found that the two ETBF clinical isolates induced E-cadherin cleavage in HT29/c1 cells and promoted colonic inflammation in C57BL/6 mice. Our results indicate that the prevalence of ETBF in polyp patients were similar in non-polyp patients suggesting that ETBF carriage does not positively correlate to polyp incidence.

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INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2014-510-000614317