지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.12
- 수록면
- 561 - 568 (8page)
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초록· 키워드
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The aim of this study was to introduce a simple method for isolation of α-lactalbumin, β-lactoglobulin and bovine serum albumin from cow’s milk, and peptides produced by enzymatic hydrolysis of α-lactalbumin, β-lactoglobulin and bovine serum albumin with alcalase. Whey protein were precipitated from whey by ammonium sulfate and, α-lactalbumin and β-lactoglobulin were isolated using Hi Prep 26/60 Sephacryl S-100 column gel filtration chromatography. Bovine serum albumin and β-lactoglobulin were isolated by Mono-Q 5/50 GL column anion exchange chromatography of the 50% Ammonium Sulfate-supernatant. Isolated whey proteins were hydrolyzed by proteolytic alcalase. Tricine SDS-PAGE and reverse-phase HPLC analyses revealed that almost hydrolyzed all the α-lactalbumin, β-lactoglobulin and bovine serum albumin with alcalase. Molecular weight of various peptides derived from alcalase hydrolysate were small molecular weight than 3.5 kDa.
목차
Abstract
Ⅰ. 서론
Ⅱ. 재료 및 방법
Ⅲ. 결과 및 고찰
Ⅳ. 결론
감사의 글
참고문헌
참고문헌 (14)
참고문헌 신청
1. [학술지(정기간행물)] - Chabance B / 1995 / Characterization of an antithrombotic peptide from κ-casein in newborn plasma after milk ingestion / British Journal of Nutrition 73 : 583 ~ 590
2. [학술지(정기간행물)] - Clementa A. / 2000 / Enzymatic protein hydrolysates in human nutrition / Trends in Food Science Technology 11 : 254 ~ 262
3. [학술지(정기간행물)] - Chicon R / 2008 / Proteolytic pattern, antigenicity, and serum immunoglobulin E binding of β-lactoglobulin hydrolysates obtained by pepsin and high-pressure treatments / Journal of Dairy Science 91 : 928 ~ 938
4. [단행본] - Fox PF. / 1989 / The milk protein system. pp 1-53. Developments in Dairy Chemistry. Vol. 4 / Elsevier Applied Science
5. [학술지(정기간행물)] - Gobbetti M / 2004 / Angiotensin Iconverting enzyme inhibitory and antimicrobial bioactive peptides / International Journal of Dairy Technology 57 : 173 ~ 188
2. [학술지(정기간행물)] - Clementa A. / 2000 / Enzymatic protein hydrolysates in human nutrition / Trends in Food Science Technology 11 : 254 ~ 262
3. [학술지(정기간행물)] - Chicon R / 2008 / Proteolytic pattern, antigenicity, and serum immunoglobulin E binding of β-lactoglobulin hydrolysates obtained by pepsin and high-pressure treatments / Journal of Dairy Science 91 : 928 ~ 938
4. [단행본] - Fox PF. / 1989 / The milk protein system. pp 1-53. Developments in Dairy Chemistry. Vol. 4 / Elsevier Applied Science
5. [학술지(정기간행물)] - Gobbetti M / 2004 / Angiotensin Iconverting enzyme inhibitory and antimicrobial bioactive peptides / International Journal of Dairy Technology 57 : 173 ~ 188
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UCI(KEPA) : I410-ECN-0101-2014-480-000601828