지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.10
- 수록면
- 249 - 254 (6page)
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초록· 키워드
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Objective: To investigate the stem cell-like characteristics of human periodontal ligament (PDL) stromal cells outgrown from orthodontically extracted premolars and to evaluate the potential for myogenic differentiation. Methods: PDL stromal cells were obtained from extracted premolars by using the outgrowth method. Cell morphological features, self-replication capability, and the presence of cell-surface markers, along with osteogenic, adipogenic, and chondrogenic differentiation, were confirmed. In addition, myogenic differentiation was induced by the use of 5-aza-2’-deoxycytidine (5-Aza) for DNA demethylation. Results: PDL stromal cells showed growth patterns and morphological features similar to those of fibroblasts. In contrast, the proliferation rates of premolar PDL stromal cells were similar to those of bone marrow and adipogenic stem cells. PDL stromal cells expressed surface markers of human mesenchymal stem cells (i.e., CD90 and CD105), but not those of hematopoietic stem cells (i.e., CD31 and CD34). PDL stromal cells were differentiated into osteogenic, adipogenic, and chondrogenic lineages. Myotube structures were induced in PDL stromal cells after 5-Aza pretreatment, but not in the absence of 5-Aza pretreatment. Conclusions: PDL stromal cells isolated from extracted premolars can potentially be a good source of postnatal stem cells for oromaxillofacial regeneration in bone and muscle.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONCLUSION
REFERENCES
참고문헌 (17)
참고문헌 신청
1. [학술대회논문] - Gronthos S / 2000 / Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo / Proc Natl Acad Sci U S A 97 : 13625 ~ 13630
2. [학술지(정기간행물)] - Seo BM / 2004 / Investigation of multipotent postnatal stem cells from human periodontal ligament / Lancet 364 : 149 ~ 155
3. [학술지(정기간행물)] - Gay IC / 2007 / Isolation and characterization of multipotent human periodontal ligament stem cells / Orthod Craniofac Res 10 : 149 ~ 160
4. [학술지(정기간행물)] - Gronthos S / 2002 / Stem cell properties of human dental pulp stem cells / J Dent Res 81 : 531 ~ 535
5. [학술지(정기간행물)] - Kobayashi M / 2009 / In vitro response of the bone marrow-derived mesenchymal stem cells seeded in a type-I collagen-glycosaminoglycan scaffold for skin wound repair under the mechanical loading condition / Mol Cell Biomech 6 : 217 ~ 227
2. [학술지(정기간행물)] - Seo BM / 2004 / Investigation of multipotent postnatal stem cells from human periodontal ligament / Lancet 364 : 149 ~ 155
3. [학술지(정기간행물)] - Gay IC / 2007 / Isolation and characterization of multipotent human periodontal ligament stem cells / Orthod Craniofac Res 10 : 149 ~ 160
4. [학술지(정기간행물)] - Gronthos S / 2002 / Stem cell properties of human dental pulp stem cells / J Dent Res 81 : 531 ~ 535
5. [학술지(정기간행물)] - Kobayashi M / 2009 / In vitro response of the bone marrow-derived mesenchymal stem cells seeded in a type-I collagen-glycosaminoglycan scaffold for skin wound repair under the mechanical loading condition / Mol Cell Biomech 6 : 217 ~ 227
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UCI(KEPA) : I410-ECN-0101-2014-515-000452105