지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: The purpose of this study was to examine the single dose toxicity with oral administration and genotoxicities of Ssanghwa-tang fermented with Lactobacillus acidophyllus.
Materials and Methods: Clinical signs, weight changes, lethal doses(LD<SUB>50</SUB>), and postmortem evaluation were determined by Globally Harmonized Classification System(GHCS) in a single-dose oral toxicity study. In vitro mammalian chromosomal aberration test was conducted with Ames test by cell proliferation suppression assessment using the cultivated CHO-K1(Chinese hamster ovary fibroblast) origins. Bacterial reversion assay was performed using Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and Escherichia coli (WP2uvrA). In vivo micronucleus test was performed using ICR mouse bone marrow.
Results: No clinical sign was observed and none of the groups with doses up to 2000 mg/kg showed significant acute oral toxicity in the single dose oral administration. None of the sample doses taken during the 6 to 18 hour groups showed significant aberrant metaphases comparing to the negative control group in the in vitro mammalian chromosomal aberration test. No evidence of mutagenicity was seen for Escherichia coli (WP2uvrA) or Salmonella typhimurium (TA98, TA100, TA1535, and TA1537). No significant increase in the frequency of micronuclei was seen in the micronucleus test.
Conclusion: These results indicate that the LD50 value of Ssanghwa-Tang fermented with Lactobacillus acidophyllus may be over 2000 mg/kg and it have no acute oral toxicity and genotoxicity.
Materials and Methods: Clinical signs, weight changes, lethal doses(LD<SUB>50</SUB>), and postmortem evaluation were determined by Globally Harmonized Classification System(GHCS) in a single-dose oral toxicity study. In vitro mammalian chromosomal aberration test was conducted with Ames test by cell proliferation suppression assessment using the cultivated CHO-K1(Chinese hamster ovary fibroblast) origins. Bacterial reversion assay was performed using Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and Escherichia coli (WP2uvrA). In vivo micronucleus test was performed using ICR mouse bone marrow.
Results: No clinical sign was observed and none of the groups with doses up to 2000 mg/kg showed significant acute oral toxicity in the single dose oral administration. None of the sample doses taken during the 6 to 18 hour groups showed significant aberrant metaphases comparing to the negative control group in the in vitro mammalian chromosomal aberration test. No evidence of mutagenicity was seen for Escherichia coli (WP2uvrA) or Salmonella typhimurium (TA98, TA100, TA1535, and TA1537). No significant increase in the frequency of micronuclei was seen in the micronucleus test.
Conclusion: These results indicate that the LD50 value of Ssanghwa-Tang fermented with Lactobacillus acidophyllus may be over 2000 mg/kg and it have no acute oral toxicity and genotoxicity.
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감사의 말씀
참고문헌
참고문헌 (15)
참고문헌 신청
1. [학술지(정기간행물)] - Shin KH / 1990 / The acute and subacute toxicities and pharmacobgical actions of Gami Ssanghwa Tang preparations / Korean Journal of Pharmacognosy 21 (2) : 179 ~ 185
2. [학술지(정기간행물)] - Lee JH / 2008 / Efficacy of fungus-fermentated Ssanghwatang on liver protection in SD male rats treated with CCl4 / Korean Journal of Oriental Medicine 14 (1) : 137 ~ 143
3. [학술지(정기간행물)] - Sohn NW / 1994 / Histochemical Study for the Effect of Ssanghwatang on the Glycogen Contents in Liver and Muscle of Rats / Journal of Herbology 9 (1) : 115 ~ 125
4. [학술지(정기간행물)] - Park WK / 1995 / Effects of Ssanghwa-tang on the Antifatigue Action and Brain Levels of Norepinephrine,Serotonin,5-hydroxyindole-acetic acid and Dopamine / The Journal of Jaehan Oriental Medical Academy 1 (1) : 130 ~ 145
5. [학술지(정기간행물)] - Kim IH / 1981 / Studies on the Antiinflammatory Activities of “Ssangwha-Tang” / Korean Journal of Pharmacognosy 12 (3) : 131 ~ 135
2. [학술지(정기간행물)] - Lee JH / 2008 / Efficacy of fungus-fermentated Ssanghwatang on liver protection in SD male rats treated with CCl4 / Korean Journal of Oriental Medicine 14 (1) : 137 ~ 143
3. [학술지(정기간행물)] - Sohn NW / 1994 / Histochemical Study for the Effect of Ssanghwatang on the Glycogen Contents in Liver and Muscle of Rats / Journal of Herbology 9 (1) : 115 ~ 125
4. [학술지(정기간행물)] - Park WK / 1995 / Effects of Ssanghwa-tang on the Antifatigue Action and Brain Levels of Norepinephrine,Serotonin,5-hydroxyindole-acetic acid and Dopamine / The Journal of Jaehan Oriental Medical Academy 1 (1) : 130 ~ 145
5. [학술지(정기간행물)] - Kim IH / 1981 / Studies on the Antiinflammatory Activities of “Ssangwha-Tang” / Korean Journal of Pharmacognosy 12 (3) : 131 ~ 135
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UCI(KEPA) : I410-ECN-0101-2014-519-000285046