지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: The aim of this investigation was to evaluate the efficacy of KHchunggan-tang aqueous extract on the experimental nonalcoholic fatty liver disease(NAFLD) induced by palmitate.
Materials and Methods: To generate a cellular model of NAFLD, we used HepG2 cells, a human hepatoma cell line, treated with 0.5 mM palmitate. By this cellular model, effects of KHchunggan-tang aqueous extract were evaluated. Intracellular lipid accumulation, free radical formation, and apoptosis were detected by Nile red staining, 2’,7’-dichloroflourescin diacetate(H2DCF-DA), and 4’,6-diamidino-2-phenylindole(DAPI)/propidium iodide(PI) staining, respectively. Some proteins related with NAFLD were determined by western blot.
Results: Typical pathological features of NAFLD occurred in the cellular model. Palmitate increased the levels of intracellular lipid vacuoles, decreased cell viability, and increased apoptosis. Palmitate increased free radical formation and lipid peroxidation, too. However, KHchunggan-tang aqueous extract reduced palmitate-induced pathologic features, i.e. steatosis, free radical formation, and apoptosis. In addition, KHchunggan-tang aqueous extract suppressed palmitate-activated c-Jun N-terminal kinase(JNK) signaling, and SP600125, a JNK inhibitor, significantly reversed the palmitate-induced pathologic changes as KHchunggan-tang aqueous extract. It means that the signaling pathway other than JNK can be involved in the KHchunggan-tang mediated cellular protection of palmitate-treated Hep G2 cells.
Conclusions: These results suggest that KHchunggan-tang aqueous extract has hepatoprotective effects on NAFLD with combined properties in cellular steatosis, ROS production, and cytoprotection, and thus may have valuable clinical applications for treatment of this chronic liver disease.
Materials and Methods: To generate a cellular model of NAFLD, we used HepG2 cells, a human hepatoma cell line, treated with 0.5 mM palmitate. By this cellular model, effects of KHchunggan-tang aqueous extract were evaluated. Intracellular lipid accumulation, free radical formation, and apoptosis were detected by Nile red staining, 2’,7’-dichloroflourescin diacetate(H2DCF-DA), and 4’,6-diamidino-2-phenylindole(DAPI)/propidium iodide(PI) staining, respectively. Some proteins related with NAFLD were determined by western blot.
Results: Typical pathological features of NAFLD occurred in the cellular model. Palmitate increased the levels of intracellular lipid vacuoles, decreased cell viability, and increased apoptosis. Palmitate increased free radical formation and lipid peroxidation, too. However, KHchunggan-tang aqueous extract reduced palmitate-induced pathologic features, i.e. steatosis, free radical formation, and apoptosis. In addition, KHchunggan-tang aqueous extract suppressed palmitate-activated c-Jun N-terminal kinase(JNK) signaling, and SP600125, a JNK inhibitor, significantly reversed the palmitate-induced pathologic changes as KHchunggan-tang aqueous extract. It means that the signaling pathway other than JNK can be involved in the KHchunggan-tang mediated cellular protection of palmitate-treated Hep G2 cells.
Conclusions: These results suggest that KHchunggan-tang aqueous extract has hepatoprotective effects on NAFLD with combined properties in cellular steatosis, ROS production, and cytoprotection, and thus may have valuable clinical applications for treatment of this chronic liver disease.
목차
서론
재료와 방법
결과
고찰 및 결론
감사의 글
참고문헌
참고문헌 (33)
참고문헌 신청
1. [단행본] - Bacon BR / 2008 / Genetic, metabolic, and infiltrative diseases affecting the liver In Harrison’s principles of internal medicine / NewYork:McGraw-Hill : 1980 ~ 1983
2. [학술지(정기간행물)] - Angulo P / 2002 / Nonalcoholic fatty liver disease / N Engl J Med 346 : 1221 ~ 1231
3. [학술지(정기간행물)] - Marchesini G / 1999 / Association of nonalcoholic fatty liver disease with insulin resistance / The American journal of medicine 107 : 450 ~ 455
4. [학술지(정기간행물)] - Sung TS / 1994 / Effect of Cnidi rhizoma boiling extract solution on enzyme and hormone of plasma, and liver in the fatted rats induced by high fat dietary / Korean J Food & Nutrition 7 (2) : 108 ~ 113
5. [학술지(정기간행물)] - 윤경수 / 2009 / NASH 동물모델에서 인진청간탕과 인진사령산의 효과규명 / 대한한방내과학회지 30 (2) : 410 ~ 421
2. [학술지(정기간행물)] - Angulo P / 2002 / Nonalcoholic fatty liver disease / N Engl J Med 346 : 1221 ~ 1231
3. [학술지(정기간행물)] - Marchesini G / 1999 / Association of nonalcoholic fatty liver disease with insulin resistance / The American journal of medicine 107 : 450 ~ 455
4. [학술지(정기간행물)] - Sung TS / 1994 / Effect of Cnidi rhizoma boiling extract solution on enzyme and hormone of plasma, and liver in the fatted rats induced by high fat dietary / Korean J Food & Nutrition 7 (2) : 108 ~ 113
5. [학술지(정기간행물)] - 윤경수 / 2009 / NASH 동물모델에서 인진청간탕과 인진사령산의 효과규명 / 대한한방내과학회지 30 (2) : 410 ~ 421
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UCI(KEPA) : I410-ECN-0101-2014-519-000285071