지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Background: Most antitumor agents have the side effect of chemotherapy-induced peripheral neuropathy (CIPN). Cancer patients who take antitumor agents suffer from CIPN, but there is no known treatment for it. Unlike the central nerve system, the peripheral nerve can self-repair, and the Schwann cell takes this mechanism.
Objectives: In this study, we researched the effect of YideungJetong-Tang (YJT) extract on taxol-induced sciatic nerve damage, through in vitro and in vivo experiments. Also, we studied the effect of YJT extract on neurite recovery and anti-inflammatory effect after compression injury of sciatic nerve in vivo.
Methods: Vehicle, taxol and taxol+YJT were respectively applied on sciatic nerve cells of rat in vitro, then the cells were cultured. The sciatic nerve cells and Schwann cells were then observed using Neurofilament 200, Hoechst, β-tubulin, S-100β, caspase-3 and phospho-Erk1/2.
CIPN was induced by taxol into the sciatic nerve of rat in vivo, then YJT extract was taken orally. The axons, Schwann cells and neurites of the DRG sensory nerve were then observed using Neurofilament 200, β-tubulin, Hoechst, S-100β, phospho-Erk1/2 and caspase-3.
YJT was taken orally after sciatic nerve compression injury, and the changes in axon of the sciatic nerve, Schwann cells and TNF-α concentration were observed.
Results: The taxol and YJT treated group showed significant effects on Schwann cell recovery, neurite growth and recovery. In vivo, YJT compared with control group showed Schwann cell structural improvement and axons recovering effect after taxol-induced Schwann cell damage. After sciatic nerve compression injury, recovery of distal axon, changes of Schwann cell distribution, and anti-inflammatory response were observed in the YJT.
Conclusions: Through this study, we found that after taxol-induced neurite damage of sciatic nerve in vivo and in vitro, YJT had significant effects on sciatic nerve growth and Schwann cell structural improvement. In vivo, YJT improved recovery of distal axons and Schwann cells and had an anti-inflammatory effect.
Objectives: In this study, we researched the effect of YideungJetong-Tang (YJT) extract on taxol-induced sciatic nerve damage, through in vitro and in vivo experiments. Also, we studied the effect of YJT extract on neurite recovery and anti-inflammatory effect after compression injury of sciatic nerve in vivo.
Methods: Vehicle, taxol and taxol+YJT were respectively applied on sciatic nerve cells of rat in vitro, then the cells were cultured. The sciatic nerve cells and Schwann cells were then observed using Neurofilament 200, Hoechst, β-tubulin, S-100β, caspase-3 and phospho-Erk1/2.
CIPN was induced by taxol into the sciatic nerve of rat in vivo, then YJT extract was taken orally. The axons, Schwann cells and neurites of the DRG sensory nerve were then observed using Neurofilament 200, β-tubulin, Hoechst, S-100β, phospho-Erk1/2 and caspase-3.
YJT was taken orally after sciatic nerve compression injury, and the changes in axon of the sciatic nerve, Schwann cells and TNF-α concentration were observed.
Results: The taxol and YJT treated group showed significant effects on Schwann cell recovery, neurite growth and recovery. In vivo, YJT compared with control group showed Schwann cell structural improvement and axons recovering effect after taxol-induced Schwann cell damage. After sciatic nerve compression injury, recovery of distal axon, changes of Schwann cell distribution, and anti-inflammatory response were observed in the YJT.
Conclusions: Through this study, we found that after taxol-induced neurite damage of sciatic nerve in vivo and in vitro, YJT had significant effects on sciatic nerve growth and Schwann cell structural improvement. In vivo, YJT improved recovery of distal axons and Schwann cells and had an anti-inflammatory effect.
목차
서론
연구대상 및 방법
결과
고찰
참고문헌
참고문헌 (36)
참고문헌 신청
1. [학술지(정기간행물)] - Scholz J / 2005 / Blocking caspase activity prevents transsynaptic neuronal apoptosis and the loss of inhibition in lamina II of the dorsal horn after peripheral nerve injury / Journal of Neuroscience 25 (32) : 7317 ~ 7323
2. [학술지(정기간행물)] - Fu SY / 1997 / The cellular and molecular basis of peripheral nerve regeneration / Molecular Neurobiology 14 (1) : 67 ~ 116
3. [단행본] - 손장원 / 2010 / Harrison's 내과학 / 대한내과학회 : 3185 ~ 3187
4. [학술지(정기간행물)] - Rowinsky EK / 1993 / Clinical toxicities encountered with paclitaxel (Taxol) / Seminars in Oncology 20 (4) : 1 ~ 15
5. [학술지(정기간행물)] - Carozzi VA / 2010 / Neurophysiological and neuropathological characterization of new murine models of chemotherapy-induced chronic peripheral neuropathies / Experimental Neurology 226 (2) : 301 ~ 309
2. [학술지(정기간행물)] - Fu SY / 1997 / The cellular and molecular basis of peripheral nerve regeneration / Molecular Neurobiology 14 (1) : 67 ~ 116
3. [단행본] - 손장원 / 2010 / Harrison's 내과학 / 대한내과학회 : 3185 ~ 3187
4. [학술지(정기간행물)] - Rowinsky EK / 1993 / Clinical toxicities encountered with paclitaxel (Taxol) / Seminars in Oncology 20 (4) : 1 ~ 15
5. [학술지(정기간행물)] - Carozzi VA / 2010 / Neurophysiological and neuropathological characterization of new murine models of chemotherapy-induced chronic peripheral neuropathies / Experimental Neurology 226 (2) : 301 ~ 309
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UCI(KEPA) : I410-ECN-0101-2014-519-000272698