지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한외과학회 Annals of Surgical Treatment and Research Journal of the Korean Surgical Society Vol.84 No.4
- 발행연도
- 2013.4
- 수록면
- 202 - 208 (7page)
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초록· 키워드
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Purpose: Primary mammalian hepatocytes largely retain their liver-specific functions when they are freshly derived from donors. However, long-term cultures of functional hepatocytes are difficult to establish. To increase the longevity and maintain the differentiated functions of hepatocytes in primary culture, cells can be cultured in a sandwich configuration of collagen. In such a configuration, hepatocytes can be cultured for longer periods compared with cultures on single layers of collagen. However, research regarding mouse hepatocytes in sandwich culture is lacking. Methods: Primary mouse hepatocytes were sandwiched between two layers of collagen to maintain the stability of their liver-specific functions. After gelation, 2 mL of hepatocyte culture medium was applied. Results: After 24 hours, 5, 10 days of culture, the collagen gel sandwich maintained the cellular border and numbers of bile canaliculi more efficiently than a single collagen coating in both high and low density culture dishes. Reverse transcription-polymerase chain reaction analysis of alpha-1-antitrypsin (AAT), hepatocyte nuclear factor 4 alpha (HNF4A), alphafetoprotein, albumin, tryptophan oxygenase (TO), the tyrosine aminotransferase gene, glucose-6-phosphatase, glyceraldehyde-3- phosphate dehydrogenase for mouse primary hepatocytes cultured on collagen coated dishes and collagen gels showed superior hepatocyte-related gene expression in cells grown using the collagen gel sandwich culture system. AAT, HNF4A, albumin, TO were found to be expressed in mouse hepatocytes cultured on collagen gels for 5 and 10 days. In contrast, mouse hepatocytes grown on collagen-coated dishes did not express these genes after 5 and 10 days of culture. Conclusion: The collagen gel sandwich method is suitable for primary culture system of adult mouse hepatocytes.
목차
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (13)
참고문헌 신청
1. [학술지(정기간행물)] - Wang YJ / 2004 / Primary hepatocyte culture in collagen gel mixture and collagen sandwich / World J Gastroenterol 10 : 699 ~ 702
2. [학술지(정기간행물)] - Swift B / 2010 / Influence of seeding density and extracellular matrix on bile Acid transport and mrp4 expression in sandwich-cultured mouse hepatocytes / Mol Pharm 7 : 491 ~ 500
3. [학술지(정기간행물)] - Mathijs K / 2009 / Assessing the metabolic competence of sandwich-cultured mouse primary hepatocytes / Drug Metab Dispos 37 : 1305 ~ 1311
4. [학술지(정기간행물)] - Martinez SM / 2010 / Evaluation of an in vitro toxicogenetic mouse model for hepatotoxicity / Toxicol Appl Pharmacol 249 : 208 ~ 216
5. [학술지(정기간행물)] - Berthiaume F / 1999 / Isolation and long-term maintenance of adult rat hepatocytes in culture / Methods Mol Med 18 : 447 ~ 456
2. [학술지(정기간행물)] - Swift B / 2010 / Influence of seeding density and extracellular matrix on bile Acid transport and mrp4 expression in sandwich-cultured mouse hepatocytes / Mol Pharm 7 : 491 ~ 500
3. [학술지(정기간행물)] - Mathijs K / 2009 / Assessing the metabolic competence of sandwich-cultured mouse primary hepatocytes / Drug Metab Dispos 37 : 1305 ~ 1311
4. [학술지(정기간행물)] - Martinez SM / 2010 / Evaluation of an in vitro toxicogenetic mouse model for hepatotoxicity / Toxicol Appl Pharmacol 249 : 208 ~ 216
5. [학술지(정기간행물)] - Berthiaume F / 1999 / Isolation and long-term maintenance of adult rat hepatocytes in culture / Methods Mol Med 18 : 447 ~ 456
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UCI(KEPA) : I410-ECN-0101-2014-490-003019533