Therapeutic potentials occurring during the early differentiation process of mesenchymal stem cells in a rats model with thioacetamide - induced liver fibrosis

Sang-Tae Choi; Shin Hwang; Hea-Nam Hong; You-Jin Won; Chul-Soo Ahn; Tae-Yong Ha; Gi-Won Song; Dong-Hwan Jung; Gil-Chun Park; Sung-Gyu Lee

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자료유형: 학술저널

저자정보: Sang-Tae Choi (Gachon University Gil Hospital, Incheon) Shin Hwang (University of Ulsan College of Medicine) Hea-Nam Hong (University of Ulsan College of Medicine) You-Jin Won (University of Ulsan College of Medicine) Chul-Soo Ahn (University of Ulsan College of Medicine) Tae-Yong Ha (Asan Medical Center) Gi-Won Song (University of Ulsan College of Medicine) Dong-Hwan Jung (University of Ulsan College of Medicine) Gil-Chun Park (University of Ulsan College of Medicine) Sung-Gyu Lee (University of Ulsan College of Medicine)

저널정보: 한국간담췌외과학회 Annals of Hepato-Biliary-Pancreatic Surgery 한국간담췌외과학회지 제17권 제1호

발행연도: 2013.3

수록면: 21 - 33 (13page)

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Backgrounds/Aims: Mesenchymal stem cells (MSCs) have the capacity to differentiate into hepatocytes, The purpose of this study is to investigate the MSCs’ differentiation process and therapeutic potentials by comparing isolated MSCs with HGF-treated MSCs in rat’s model with thiacetamide (TAA)-induced cirrhosis. Methods: Male Sprague-Dawley (SD) rats, weighing 100-150 g were used in this study. To induce liver fibrosis, recipient rats were taken with 0.04% thioacetamide (TAA) in the drinking water (400 mg TAA/L) for 8 weeks. The rats underlying liver cirrhosis were divided into 3 groups according to the transplanted materials, compared to normal saline as control (I) and isolated MSCs (II) HGF-treated MSCs. Results: Severe hepatic fibrosis and hepatocyte destruction were detected in the control group. Less hepatic cirrhosis and collagen formation, more hepatocyte regeneration and glycogen storage were detected in isolated MSCs compared to HGF-treated MSCs group, Distribution of red autofluorescence is mainly localized near the sinusoids in isolated MSCs, scattered away the sinusoids in HGF-treated MSCs group. MSCs transdifferentiated into CK-19 postive Oval cells and then to albulmin-producing hepatocytes, HGF treated MSCs differentiated into hepatocyte without the intermediate oval cells phase. HGF treated MSCs became the CK18-positive, MSCs became CD 90-positive. Conclusions: Significant hepatocyte differentiation occurred in not HGF-treated MSCs but isolated MSCs group unexpectedly. These results suggest that the beneficial effect of MSCs on in rat’s model with TAA-induced cirrhosis may occur during early differentiation course of MSCs. Mature hepatocyte itself has a little effect on the accelerated differentiation and functional capacity of hepatic lineage cell-line.

#Mesenchymal stem cell #Hepatocyte #Differentiation #Liver cirrhosis #Rat model #Thioacetamide

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Sang-Tae Choi

소속기관 Gachon University Gil Hospital, Incheon

주요연구분야 의약학 > 임상의학 > 일반 외과학

논문수 3 이용수 21

Shin Hwang