T Regulatory Cell Responses to Immunization with a Soluble Egg Antigen in Schistosoma mansoni-Infected Mice

Eman El-Ahwany; Ibrahim Rabia Bauiomy; Faten Nagy; Rabab Zalat; Ola Mahmoud; Suher Zada

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저자정보: Eman El-Ahwany (Theodor Bilharz Research Institute) Ibrahim Rabia Bauiomy (Theodor Bilharz Research Institute) Faten Nagy (Theodor Bilharz Research Institute) Rabab Zalat (Theodor Bilharz Research Institute) Ola Mahmoud (Theodor Bilharz Research Institute) Suher Zada (American University in Cairo)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.50 No.1

발행연도: 2012.2

수록면: 29 - 35 (7page)

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The aim of the study is to characterize the phenotypes of CD4+ CD25+ T regulatory cells within the liver granulomas and association with both Foxp-3 gene expression and splenic cytokines. Naive C57BL/6 mice were intravenously injected with multiple doses of the soluble egg antigen (SEA) 7 days before cercarial infection. The immunized and infected control groups were sacrificed 8 and 16 weeks post-infection (PI). Histopathology, parasitological parameters, splenic phenotypes for T regulatory cells, the FOXP-3 expression in hepatic granuloma using real-time PCR, and the associated splenic cytokines were studied. Histopathological examination of the liver revealed remarkable increase in degenerated ova within hepatic granuloma which decreased in diameter at weeks 8 and 16 PI (P<0.01). The percentage of T regulatory cells (CD4+ CD25+) increased significantly (P<0.01) in the immunized group compared to the infected control at weeks 8 and 16 PI. The FOXP-3 expression in hepatic granulomas increased from 10 at week 8 to 30 fold at week 16 PI in the infected control group. However, its expression in the immunized group showed an increase from 30 at week 8 to 70 fold at week 16 PI. The splenic cytokine levels of pro-inflammatory cytokines, IFN-γ, IL-4, and TNF-α, showed significant decreases (P<0.05) compared to the infected control group. In conclusion, the magnitude and phenotype of the egg-induced effects on T helper responses were found to be controlled by a parallel response within the T regulatory population which provides protection in worm parasite-induced immunopathology.

#Schistosoma mansoni #T regulatory cell #soluble egg antigen #Foxp-3 gene

참고문헌 (44)

참고문헌 신청

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