Fasciola gigantica Fatty Acid Binding Protein (FABP) as a Prophylactic Agent against Schistosoma mansoni Infection in CD1 Mice

Ibrahim Rabia Aly; M. Diab; A. M. El-Amir; M. Hendawy; S. Kadry

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자료유형: 학술저널

저자정보: Ibrahim Rabia Aly (Theodore Bilharz Research Institute) M. Diab (Theodore Bilharz Research Institute) A. M. El-Amir (Cairo University) M. Hendawy (Theodore Bilharz Research Institute) S. Kadry (Cairo University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.50 No.1

발행연도: 2012.2

수록면: 37 - 43 (7page)

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Although schistosomicidal drugs and other control measures exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. In this study, native fatty acid binding protein (FABP) from Fasciola gigantica was purified from the adult worm’s crude extract by saturation with ammonium sulphate followed by separation on DEAE-Sephadex A-50 anion exchange chromatography and gel filtration using Sephacryl HR-100, respectively. CD1 mice were immunized with the purified, native F. gigantica FABP in Freund’s adjuvant and challenged subcutaneously with 120 Schistosoma mansoni cercariae. Immunization of CD1 mice with F. gigantica FABP has induced heterologous protection against S. mansoni, evidenced by the significant reduction in mean worm burden (72.3%), liver and intestinal egg counts (81.3% and 80.8%, respectively), and hepatic granuloma counts (42%). Also, it elicited mixed IgG1/IgG2b immune responses with predominant IgG1 isotype, suggesting that native F. gigantica FABP is mediated by a mixed Th1/Th2 response. However, it failed to induce any significant differences in the oogram pattern or in the mean granuloma diameter. This indicated that native F. gigantica FABP could be a promising vaccine candidate against S. mansoni infection.

#Schistosoma mansoni #Fasciola gigantica #FABP #heterologous protection #Th1/Th2 immunity

참고문헌 (34)

참고문헌 신청

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